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应用
生化/生理作用
外形
缓冲水溶液包含：40 mM Tris-HCl，pH 8.0，110 mM NaCl、2.2 mM KCl、8 mM 咪唑、0.04% Tween-20 和 20% 甘油
分析说明
本产品可以在含有 50 mM HEPES （pH=7.4）、150 mM NaCl、5 mM CaCl2、5 mM (Gly)3、25 mM Abz/Dnp 底物和转肽酶 A 的反应缓冲液 (50 μl) 中 在 30°C 下保温 30 分钟进行分析。 荧光强度在 Ex320nm/Em420nm 处测量。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Hongyuan Mao et al.
Journal of the American Chemical Society, 126(9), 2670-2671 (2004-03-05)
Sortase (SrtA), a transpeptidase from Staphylococcus aureus, catalyzes a cell-wall sorting reaction at an LPXTG motif by cleaving between threonine and glycine and subsequently joining the carboxyl group of threonine to an amino group of pentaglycine on the cell wall
John M Antos et al.
Journal of the American Chemical Society, 130(48), 16338-16343 (2008-11-08)
A general chemoenzymatic method for the site-specific attachment of lipids to protein substrates is described. Sortase A is used to append short lipid-modified oligoglycine peptides to the C terminus of protein substrates bearing a five amino acid sortase A recognition
Maximilian W Popp et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(8), 3169-3174 (2011-02-08)
Recombinant protein therapeutics often suffer from short circulating half-life and poor stability, necessitating multiple injections and resulting in limited shelf-life. Conjugation to polyethylene glycol chains (PEG) extends the circulatory half-life of many proteins, but the methods for attachment often lack
Stephan Pritz et al.
The Journal of organic chemistry, 72(10), 3909-3912 (2007-04-17)
Sortase A is a transpeptidase that cleaves at a pentapeptide-motif and subsequently transfers the acyl component to a nucleophile containing N-terminal oligoglycines. We investigate the reaction conditions of the sortase-mediated ligation and demonstrate a useful application by the synthesis of
S K Mazmanian et al.
Science (New York, N.Y.), 285(5428), 760-763 (1999-07-31)
Surface proteins of Gram-positive bacteria are linked to the bacterial cell wall by a mechanism that involves cleavage of a conserved Leu-Pro-X-Thr-Gly (LPXTG) motif and that occurs during assembly of the peptidoglycan cell wall. A Staphylococcus aureus mutant defective in
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