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主要文件

安全信息

SRP0166

Sigma-Aldrich

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

别名:

DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

human

重组

expressed in baculovirus infected insect cells

方案

≥90% (SDS-PAGE)

表单

aqueous solution

分子量

27 kDa (DNMT3L)
59 kDa (DNMT3B)

包装

pkg of 10 μg

浓度

>0.02 mg/mL

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... DNMT3B(1789)

一般描述

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

应用

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

生化/生理作用

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

外形

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

? DNMT3B4-del Contributes to Aberrant DNA Methylation Patterns in Lung Tumorigenesis
Mark Z. Ma
EBioMedicine, 2(10), 1340-1350 (2015)
Association between single-nucleotide polymorphisms of DNMT3L and infertility with azoospermia in Chinese men.
Huang JX
Reproductive Biomedicine Online, 24(1), 66-71 (2012)
Associations of DNMT3B -149C>T and -2437T>A polymorphisms and lung cancer risk in Chinese population
Min Gao
World Journal of Surgical Oncology, 14, 293-293 (2016)
DNA methyltransferase 3B -149C/T
polymorphism and the risk of laryngeal
squamous cell carcinoma: a case-control
study
X. M. Zhang
Genetics and molecular research : GMR, 14(4), 12866-12871 (2015)
Polymorphism of DNA Methyltransferase 3b and Association with Development and Prognosis in Gastric Cancer
Chuan Wang
PLoS ONE, 10(8), e0134059-e0134059 (2015)

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