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安全信息

SHPHLIBR

Sigma-Aldrich

MISSION® LentiPlex® 人类混合shRNA完整文库

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

UNSPSC代码:
41105904
NACRES:
NA.51

质量水平

200

产品线

MISSION®

浓度

≥5x108 VP/ml (via p24 assay)

技术

capture ELISA: 5 x 108 TU/mL using p24

运输

dry ice

储存温度

−70°C

相关类别

一般描述

MISSION LentiPlex混合文库结合MISSION TRC shRNA集合和Sigma慢病毒生产经验的优势，为研究人员提供覆盖全基因组的RNAi筛选。LentiPlex混合系统对非分裂和原代细胞系的递送和长期基因沉默能力更强，探索潜能无限。方便易用的形式让研究人员可在最少的试剂、时间或固定设备投入下，实现快速的全基因组RNAi筛选。

MISSION LentiPlex Complete Human shRNA Pooled Library（人类shRNA混合完整文库）是通过专有流程生产的覆盖全基因组的慢病毒池（慢病毒载体shRNA文库）。文库共有125,000多个来自TRC集合的shRNA构建体，针对2万多个人类基因。每个文库在放行前都经过部分shRNA检测，确保稳健的文库覆盖。文库为即用型慢病毒，滴度在5 x 108 TU/ml以上（p24蛋白检测法），预分为16个亚池，每个亚池约含6,000-8,000个shRNA构建体。另提供下游目标基因鉴定用的扩增和测序引物。

如需关于样品数据和使用等的更多信息，请访问我们的 LentiPlex技术详情页面。

其他说明

MISSION LentiPlex人类shRNA混合文库由货号SHPH01、SHPH15、SHPH2和SHPHLIBR产品组成。从说明书表1可以了解每个文库的shRNA克隆覆盖、靶基因和亚池的大致数目。

另提供用于下游沉默基因鉴定的扩增和测序引物。

shRNA池反卷积
Sigma的反卷积(deconvolution)服务让研究人员可以轻松发现会影响shRNA混合筛选的基因。

  • 对克隆进行新一代测序可确定shRNA混合样品中特定克隆的准确数目
  • shRNA混合筛选提供全面的、重现性结果
  • 稳健统计和信息丰富的数据

更多信息可联系您当地的Sigma销售代表,或发邮件至MISSION RNAi

法律信息

慢病毒许可协议
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品--病毒，疫苗等

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number

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访问文档库

Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells.
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology

商品

Genomic DNA Prep & Deconvolution Services

Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.

shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

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实验方案

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

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