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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
MEM-148, monoclonal
形式
buffered aqueous solution
种属反应性
human
浓度
1 mg/mL
技术
FUNC: suitable
flow cytometry: suitable using (QC Tested)
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... ITGB2(3689)
相关类别
一般描述
ITGB2 (integrin β2) encodes common β-chain of the β2 integrin family, known as cluster of differentiation 18 (CD18). CD18, integrin beta2 subunit, forms heterodimers with fourtypes of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2(CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2(CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediatedby the integrin is a composite of the functions of its individual subunits.These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesiondeficiency-1; severe reduction of CD18 expression leads to the development of apsoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella)haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediatedcytolysis. CD18 is primarily expressed in blood. The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. In human chromosome, the gene CD18 is localized on 21q22.3.
免疫原
Peripheral blood mononuclear cells
应用
The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes. Flow cytometry: Recommended dilution: 8 μg/ml. The antibody MEM-148 is an excellent marker of activated myeloid cells. Western blotting: Non-reducing conditions
生化/生理作用
Cluster of differentiation (CD18) is involved in adhesion of leukocytes to the endothelium and its migration to the site of inflammation. Lower expression of CD18 is critical because of its involvement in all the heterodimeric complex and controls the expression of α subunit. CD18 is a part of intracellular signalling molecule, signals through phosphatidylinositol 3 kinase (PI3K) and tyrosine kinase. Mutations in ITGB2 causes autosomal recessive leukocyte adhesion deficiency type 1 disorder, characterized by frequent bacterial and fungal infections. Polymorphism in ITGB2 is associated with chronic lymphocytic leukemia (CLL). Mutations in CD18 causes Hirschsprung′s disease (HSCR)-associated enterocolitis (HAEC) and chronic colitis. ITGB2 is upregulated in atherosclerotic plaque.
特点和优势
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外形
Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Kindlin 3 (FERMT3) is associated with unstable atherosclerotic plaques, anti-inflammatory type II macrophages and upregulation of beta-2 integrins in all major arterial beds
Atherosclerosis, 242(1), 145-154 (2015)
Phagocyte immunodeficiencies and their infections
The Journal of Allergy and Clinical Immunology, 113(4), 620-626 (2004)
CD11/18
Encyclopedia of Respiratory Medicine, 338-343 (2006)
Molecular characterization of leukocyte adhesion deficiency-I in Indian patients: identification of 9 novel mutations
Blood Cells, Molecules and Diseases, 54(3), 217-223 (2015)
Evaluating a CLL susceptibility variant in ITGB2 in families with multiple subtypes of hematological malignancies
Blood, 130(1), 86-88 (2017)
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