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NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
immunofluorescence
immunohistochemistry
western blot
immunofluorescence
immunohistochemistry
western blot
Species reactivity:
human, mouse
Citations:
7
Technique(s):
ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
Uniprot accession no.:
产品名称
Anti-MYF5 antibody produced in rabbit, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 28 kDa
species reactivity
human, mouse
concentration
~1 mg/mL
technique(s)
ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MYF5(4617)
Application
Anti-MYF5 antibody produced in rabbit has been used in western blotting and immunohistochemistry.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description
Myogenic factor 5 (MYF5) regulates the determination of skeletal myoblasts during embryonic development. Inactivation of MYF5 in mice results in abnormal development of the ribs, which eventually leads to perinatal death. The gene encoding this protein is localized on human chromosome 12q21.
Immunogen
The antiserum was produced against synthesized peptide derived from human MYF5.
Immunogen Range: 21-70
Immunogen Range: 21-70
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Assignment of the human myogenic factors 5 and 6 (MYF5, MYF6) gene cluster to 12q21 by in situ hybridization and physical mapping of the locus between D12S350 and D12S106
Cupelli L, et al.
Cell, 72(2-3), 250-251 (1996)
Sonic hedgehog is required for neural crest-dependent patterning of the intrinsic tongue musculature
Shigeru O, et al.
bioRxiv, 154443-154443 (2017)
Myogenic potential of mouse embryonic stem cells lacking functional Pax7 tested in vitro by 5-azacitidine treatment and in vivo in regenerating skeletal muscle
Anita H, et al.
European Journal of Cell Biology, 96(1), 47-60 (2017)
Daniela Calzia et al.
Journal of cellular physiology, 235(4), 3508-3518 (2019-09-25)
Muscle loss is a major problem for many in lifetime. Muscle and bone degeneration has also been observed in individuals exposed to microgravity and in unloading conditions. C2C12 myoblst cells are able to form myotubes, and myofibers and these cells
T Braun et al.
Cell, 71(3), 369-382 (1992-10-30)
The Myf-5 gene, a member of the myogenic basic HLH factor family, has been inactivated in mice after homologous recombination in ES cells. Mice lacking Myf-5 were unable to breathe and died immediately after birth, owing to the absence of
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