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Merck
CN

SAB4501943

Anti-MYF5 antibody produced in rabbit

affinity isolated antibody

别名:

MYF5, myogenic factor 5

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关于此项目

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA
immunofluorescence
immunohistochemistry
western blot
Species reactivity:
human, mouse
Citations:
7
Technique(s):
ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
Uniprot accession no.:
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产品名称

Anti-MYF5 antibody produced in rabbit, affinity isolated antibody

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 28 kDa

species reactivity

human, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... MYF5(4617)

Application

Anti-MYF5 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

Myogenic factor 5 (MYF5) regulates the determination of skeletal myoblasts during embryonic development. Inactivation of MYF5 in mice results in abnormal development of the ribs, which eventually leads to perinatal death. The gene encoding this protein is localized on human chromosome 12q21.

Immunogen

The antiserum was produced against synthesized peptide derived from human MYF5.

Immunogen Range: 21-70

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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存储类别

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Assignment of the human myogenic factors 5 and 6 (MYF5, MYF6) gene cluster to 12q21 by in situ hybridization and physical mapping of the locus between D12S350 and D12S106
Cupelli L, et al.
Cell, 72(2-3), 250-251 (1996)
Sonic hedgehog is required for neural crest-dependent patterning of the intrinsic tongue musculature
Shigeru O, et al.
bioRxiv, 154443-154443 (2017)
Myogenic potential of mouse embryonic stem cells lacking functional Pax7 tested in vitro by 5-azacitidine treatment and in vivo in regenerating skeletal muscle
Anita H, et al.
European Journal of Cell Biology, 96(1), 47-60 (2017)
Daniela Calzia et al.
Journal of cellular physiology, 235(4), 3508-3518 (2019-09-25)
Muscle loss is a major problem for many in lifetime. Muscle and bone degeneration has also been observed in individuals exposed to microgravity and in unloading conditions. C2C12 myoblst cells are able to form myotubes, and myofibers and these cells
T Braun et al.
Cell, 71(3), 369-382 (1992-10-30)
The Myf-5 gene, a member of the myogenic basic HLH factor family, has been inactivated in mice after homologous recombination in ES cells. Mice lacking Myf-5 were unable to breathe and died immediately after birth, owing to the absence of

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