生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 38 kDa
种属反应性
rat, mouse, human
浓度
~1 mg/mL
技术
ELISA: 1:20000
immunofluorescence: 1:100-1:500
western blot: 1:500-1:1000
NCBI登记号
UniProt登记号
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... SAE1(10055)
一般描述
E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle.
免疫原
The antiserum was produced against synthesized peptide derived from human AOS1.
Immunogen Range: 221-270
Immunogen Range: 221-270
生化/生理作用
SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2.
特点和优势
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外形
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
SUMO-activating SAE1 transcription is positively regulated by Myc.
Amente S, et al.
American Journal of Cancer Research, 2(3), 330-334 (2012)
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, et al.
Faseb Journal, 15(10), 1825-1827 (2001)
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.
Tatham MH, et al.
The Journal of Biological Chemistry, 276(38), 35368-35374 (2001)
Robert Coukos et al.
eLife, 10 (2021-08-21)
The trafficking of specific protein cohorts to correct subcellular locations at correct times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach to probe the molecular mechanisms of protein trafficking, but
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