推荐产品
生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
AK-11, monoclonal
形式
buffered aqueous solution
分子量
~56 kDa
种属反应性
mouse, chicken, rat, human
浓度
~1.0 mg/mL
技术
immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract
immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells
immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pThr450)
基因信息
human ... AKT1(207)
一般描述
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.
特异性
Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).
免疫原
Synthetic peptide corresponding to human AKT1 pThr450
应用
Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunoprecipitation
生化/生理作用
Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Novel somatic and germline mutations in intracranial germ cell tumours
Wang L, et al.
Nature, 511(7508), 241-245 (2014)
The Akt pathway in oncology therapy and beyond
Nitulescu G M, et al.
International Journal of Oncology, 53(6), 2319-2331 (2018)
Akt/PKB: one kinase, many modifications
Risso G, et al.
The Biochemical Journal, 468(2), 203-214 (2015)
Signalling specificity in the Akt pathway in breast cancer
Clark A R and Toker A
Biochemical Society Transactions, 42(5), 1349-1355 (2014)
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