产品名称
Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse, clone ALIX-1, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ALIX-1, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
flow cytometry: 5-10 μg/test using using human MOLT4 cells
immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.
immunofluorescence: 10-20 μg/mL using using human HeLa cells
immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PDCD6IP(10015)
Application
Monoclonal Anti-ALIX/PDCD6IP antibody has been used in
- immunoblotting
- immunoprecipitation
- immunofluorescence
- flow cytometry
Biochem/physiol Actions
ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).
Immunogen
peptide corresponding to an internal region of human ALIX/ PDCD6IP
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
The multiple personalities of Alix
Odorizzi G.
Journal of Cell Science, 119(15), 3025-3032 (2006)
AIP1/Alix is a binding partner of Sendai virus C protein and facilitates virus budding
Sakaguchi T, et al.
Journal of Virology, 79(14), 8933-8941 (2005)
Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
Morita E, et al.
The Embo Journal, 26(19), 4215-4227 (2007)
Xuefeng Ren et al.
Traffic (Copenhagen, Denmark), 12(10), 1282-1290 (2011-04-27)
Most membrane-enveloped viruses bud from infected cells by hijacking the host ESCRT machinery. The ESCRTs are recruited to the budding sites by viral proteins that contain short proline (Pro)-rich motifs (PRMs) known as late domains. The late domains probably evolved
Christine Chatellard-Causse et al.
The Journal of biological chemistry, 277(32), 29108-29115 (2002-05-30)
ALG-2-interacting protein X (Alix), also known as AIP1, is a cytoplasmic protein ubiquitously expressed and concentrated in phagosomes and exosomes. Alix may regulate apoptosis since it binds apoptosis-linked gene 2 (ALG-2), a Ca2+-binding protein necessary for cell death, and also
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