生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
ALIX-1, monoclonal
形式
buffered aqueous solution
种属反应性
human
浓度
~1 mg/mL
技术
flow cytometry: 5-10 μg/test using using human MOLT4 cells
immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.
immunofluorescence: 10-20 μg/mL using using human HeLa cells
immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PDCD6IP(10015)
相关类别
一般描述
Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).
免疫原
peptide corresponding to an internal region of human ALIX/ PDCD6IP
应用
Monoclonal Anti-ALIX/PDCD6IP antibody has been used in
- immunoblotting
- immunoprecipitation
- immunofluorescence
- flow cytometry
生化/生理作用
ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
The multiple personalities of Alix
Odorizzi G.
Journal of Cell Science, 119(15), 3025-3032 (2006)
AIP1/Alix is a binding partner of Sendai virus C protein and facilitates virus budding
Sakaguchi T, et al.
Journal of Virology, 79(14), 8933-8941 (2005)
Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis
Morita E, et al.
The Embo Journal, 26(19), 4215-4227 (2007)
Xuefeng Ren et al.
Traffic (Copenhagen, Denmark), 12(10), 1282-1290 (2011-04-27)
Most membrane-enveloped viruses bud from infected cells by hijacking the host ESCRT machinery. The ESCRTs are recruited to the budding sites by viral proteins that contain short proline (Pro)-rich motifs (PRMs) known as late domains. The late domains probably evolved
Christine Chatellard-Causse et al.
The Journal of biological chemistry, 277(32), 29108-29115 (2002-05-30)
ALG-2-interacting protein X (Alix), also known as AIP1, is a cytoplasmic protein ubiquitously expressed and concentrated in phagosomes and exosomes. Alix may regulate apoptosis since it binds apoptosis-linked gene 2 (ALG-2), a Ca2+-binding protein necessary for cell death, and also
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门