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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
N-GAD65, monoclonal
形式
buffered aqueous solution
分子量
antigen ~65 kDa
种属反应性
human, mouse, monkey, rat
浓度
~1.0 mg/mL
技术
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)
一般描述
Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.
应用
Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.
生化/生理作用
Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Optimized purification strategies for the elimination of non-specific products in the isolation of GAD65-specific monoclonal autoantibodies
Jiang W, et al.
F1000Research, 4 (2015)
Wei Jiang et al.
F1000Research, 4, 135-135 (2015-05-29)
Autoantibodies against antigens expressed by insulin-producing β cells are circulating in both healthy individuals and patients at risk of developing Type 1 diabetes. Recent studies suggest that another set of antibodies (anti-idiotypic antibodies) exists in this antibody/antigen interacting network to regulate
GAD65 autoantibodies and its role as biomarker of Type 1 diabetes and Latent Autoimmune Diabetes in Adults (LADA)
Towns R and Pietropaolo M
Drugs of the Future, 36(11), 847-847 (2011)
Purification and characterization of GAD65-specific monoclonal autoantibodies [version 1; referees: awaiting peer review]
Jiang W, et al.
F1000Research, 5003(7), 1VL-1VL (2015)
GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop
Fenalti G, et al.
Nature Structural and Molecular Biology, 14(4), 280-280 (2007)
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