推荐产品
生物来源
rat
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
6F7, monoclonal
表单
buffered aqueous solution
种属反应性
all
浓度
2-4 mg/mL
技术
western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
同位素/亚型
IgG1
免疫原序列
DYKDDDDK
运输
dry ice
储存温度
−20°C
一般描述
单克隆抗FLAG过氧化物酶是从生长于生物反应器中的6F7杂交瘤细胞培养上清中分离出来、与辣根过氧化物酶（HRP）偶联的单克隆抗FLAG（大鼠IgG1同种型）的免疫球蛋白纯化级分。杂交瘤6F7是FLAG肽免疫大鼠的骨髓瘤细胞和脾细胞融合产生的。
该产品可识别N端、C端和内部FLAG标签融合蛋白。特别推荐用于识别C端FLAG标签融合蛋白。
免疫原
从生长于生物反应器中的6F7杂交瘤细胞培养上清中分离出来、与辣根过氧化物酶（HRP）偶联的单克隆抗FLAG（大鼠IgG1同种型）的免疫球蛋白纯化级分。
外形
溶解在0.01M磷酸缓冲盐,pH7.4, 含有0.01%硫柳汞。
法律信息
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Claudia Isabelle Keller Valsecchi et al.
Nature, 589(7840), 137-142 (2020-11-20)
Confinement of the X chromosome to a territory for dosage compensation is a prime example of how subnuclear compartmentalization is used to regulate transcription at the megabase scale. In Drosophila melanogaster, two sex-specific non-coding RNAs (roX1 and roX2) are transcribed
Jente Stouthamer et al.
Methods in molecular biology (Clifton, N.J.), 2690, 193-204 (2023-07-14)
Interactions between extracellular domains (ECDs) are crucial for many physiological processes in the cell, most importantly perception of its environment. However, studying these often-transient interactions can be challenging. Here we describe a method that allows for in vitro detection of
W R Force et al.
The Journal of biological chemistry, 275(15), 11121-11129 (2001-02-07)
Lymphotoxin-beta receptor (LTbetaR), a member of the tumor necrosis factor receptor superfamily, is essential for the development and organization of secondary lymphoid tissue. Wild type and mutant LTbetaR containing successive truncations of the cytoplasmic domain were investigated by retrovirus-mediated gene
Jasjot Singh et al.
Nature communications, 13(1), 6212-6212 (2022-10-21)
Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in
Meidi Gu et al.
Nature immunology, 22(2), 193-204 (2021-01-06)
Metabolic reprograming toward aerobic glycolysis is a pivotal mechanism shaping immune responses. Here we show that deficiency in NF-κB-inducing kinase (NIK) impairs glycolysis induction, rendering CD8+ effector T cells hypofunctional in the tumor microenvironment. Conversely, ectopic expression of NIK promotes
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