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安全信息

SAB2702132

Sigma-Aldrich

抗-HIF1A 兔抗

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别名:
HIF1-ALPHA, MOP1, PASD8, bHLHe78
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

形式

liquid

分子量

93 kDa

种属反应性

mouse, bovine, human, rat

浓度

1 mg/mL

技术

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: 1:100-1:1000
immunoprecipitation (IP): suitable
western blot: 500-10000

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... HIF1A(3091)

免疫原

包含与 HIF1 alpha 的 657 和 826 位氨基酸相对应的序列的重组片段

应用

建议的起始稀释度如下:ICC/IF:1:100-1:1000,IHC-Fr:稀释度由测定方法确定,IHC-P:1:100-1:1000,IP:1:100-1:570, WB:1:500-1:10000,ChIP 测定法:稀释度由测定方法确定。尚未在其他应用中进行测试。最佳工作稀释液应由终端用户通过实验确定。

生化/生理作用

缺氧诱导因子-1 (HIF1)是在低氧培养的哺乳动物细胞中发现的一种转录因子,它在对缺氧的细胞和系统体内稳态反应中起着至关重要的作用。HIF1 是由 α 亚基和 β 亚基组成的异二聚体。β 亚基已被确定为芳香烃受体核转位子(ARNT)。该基因编码 HIF-1 的 α 亚基。研究表明,该基因的天然反义转录物(aHIF)的过表达与非乳头状肾癌有关。已经鉴定出编码不同同工型的两个替代转录本。[由 RefSeq 提供]

特点和优势

您可完全放心的评估我们的抗体。如果抗体在您的应用中没有发挥效果,我们将给予您完全的赔偿或更换抗体。 了解更多。

外形

1XPBS,1%BSA,20% 甘油(pH 7),添加 0.025% 的 ProClin 300 作为防腐剂。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Aquatic Chronic 3 - Skin Sens. 1

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

含少量动物源组分生物产品
常规特殊物品

分析证书(COA)

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Lehao Ren et al.
Virologica Sinica, 36(6), 1532-1542 (2021-09-15)
Viruses depend on host cellular metabolism to provide the energy and biosynthetic building blocks required for their replication. In this study, we observed that influenza A virus (H1N1), a single-stranded, negative-sense RNA virus with an eight-segmented genome, enhanced glycolysis both
Ang Luo et al.
NAR cancer, 2(2), zcaa010-zcaa010 (2020-08-04)
Mechanistic understanding of hypoxia-responsive signaling pathways provides important insights into oxygen- and metabolism-dependent cellular phenotypes in diseases. Using SILAC-based quantitative proteomics, we provided a quantitative map identifying over 6300 protein groups in response to hypoxia in prostate cancer cells and
Magdalena H Menhofer et al.
Cardiovascular research, 104(2), 303-314 (2014-09-23)
Inhibiting angiogenesis is a major approach in tumour therapy. To combat angiogenesis, the tubulin cytoskeleton has emerged as an interesting target in many pre- and clinical studies. Contrarily, the actin cytoskeleton has been largely neglected as a potential drug target
Lei Yang et al.
Molecular therapy. Nucleic acids, 20, 292-307 (2020-03-22)
Recently, the identification of several circular RNAs (circRNAs) as vital regulators of microRNAs (miRNAs) underlines the increasing complexity of non-coding RNA (ncRNA)-mediated regulatory networks. This study aimed to explore the effects of mmu_circ_0000790 on the biological behaviors of pulmonary artery
Junhua Mao et al.
American journal of physiology. Renal physiology, 307(9), F1023-F1032 (2014-07-06)
Podocytes play a key role in the formation of cellular crescents in experimental and human diseases. However, the underlying mechanisms for podocytes in promoting crescent formation need further investigation. Here, we demonstrated that mammalian target of rapamycin complex 1 (mTORC1)

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