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安全信息

S6782

Sigma-Aldrich

Superdex®

Superdex®200 Prep Grade

别名:

Size exclusion media, Superdex column

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About This Item

MDL编号:
UNSPSC代码:
23151817
NACRES:
NA.56

质量水平

形式

suspension (20% aqueous ethanol)

技术

protein array: suitable

基质

cross-linked agarose

粒径

24-44 μm

孔径

1-100 kDa MW range (dextrans)
10-600 kDa MW range (proteins)

operating pH range

1-14(short term)
3-12(long term)

储存温度

2-8°C

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一般描述

将葡聚糖凝胶基质的高选择性与交联琼脂糖的化学和物理稳定性相结合。

应用

Superdex® 可用于蛋白质层析、凝胶过滤层析、凝胶过滤介质、树脂和分离介质。Superdex® 已被用于从食用菌 猴头菇 中纯化和表征一种新型漆酶。Superdex ® 还被用于 紫荆 种子作为潜在人类肝细胞癌抑制剂的纯化和机理研究。
针对尺寸排阻色谱法的高分辨率制备分离进行了优化。列出了球状蛋白和葡聚糖的推荐分馏范围。

法律信息

Superdex is a registered trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

104.0 °F

闪点(°C)

40 °C

法规信息

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J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
Partial purification of two proteins (100 kDa and 67 kDa) cooperating in the ATP-dependent inactivation of spinach leaf nitrate reductase.
Spill, D., and Kaiser, W.M.
Planta, 192(2), 183-188 (1994)

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