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形式
liquid
用途
sufficient for 100 reactions
sufficient for 400 reactions
特点
dNTPs included
hotstart
浓度
1 units/reaction (20 μL reaction volume)
技术
qPCR: suitable
颜色
colorless
输入
purified DNA
相容性
for use with Roche LightCycler 480
检测方法
SYBR® Green
运输
wet ice
储存温度
−20°C
一般描述
SYBR Green Taq ReadyMix可用于单个产品实时扩增实验，也可用于评价产生荧光标记探针之前的引物序列。 不建议将荧光标记探针与SYBR Green I染料一起使用。
应用
特点和优势
- 方便的2×浓缩预混液，专门设计用于毛细管仪器，例如Roche LightCycler®，是高通量应用的理想选择
- 提高特异性&目标产量-JumpStart™ Taq聚合酶可防止非特异性产物，从而获得更准确的CT值并改善定量
- 该预混液可确保反应间一致性
- 缩短了制备时间，并降低了多个移液步骤造成的污染风险
- SYBR® Green I荧光染料特异性结合双链DNA，低价、易用、灵敏，是任意DNA序列定量分析的理想选择
包装
100RXN包装为1 X 1 mL
400RXN包装为1 X 4 mL
原理
JumpStart Taq抗体在室温下能够使DNA聚合酶失活。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全被激活。JumpStart Taq DNA聚合酶防止非特异性扩增，能够获得更为准确的CT值。
为制备反应，将10 μL ReadyMix加入引物、模板和水中，使最终反应体积为20 μL。
单位定义
法律信息
相关产品
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
商品
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸
实验方案
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
我们的SYBR Green qPCR实验方案是一种旨在准确定量基因表达和RT-PCR反应的的方法
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
相关内容
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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