推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
buffered aqueous solution
技术
indirect ELISA: 1:50,000-1:100,000
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
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一般描述
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. Antiserum is determined to be immunospecific for sheep IgG by immunoelectrophoresis (IEP) versus sheep IgG and normal sheep serum. Identity and purity of the antibody is established by immunoelectrophoresis (IEP). Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum result in single arcs of precipitation in the γ region.
特异性
Binds all sheep Igs
免疫原
purified sheep IgG
应用
Anti-Sheep IgG (whole molecule) antibody produced in rabbit has been used in:
- Enzyme-linked immunosorbent assay (ELISA)
- Immunoprecipitation
- labelling electrodes in immunosensor systems
生化/生理作用
Digestion of IgG by papain generates fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallisable (fc). IgG antibody has enormous therapeutic potential and the Fc is involved in the development of therapeutic antibody.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Journal of cell science, 125(Pt 22), 5453-5466 (2012-09-08)
The chitin synthase that makes the primary septum during cell division in budding yeasts is an important therapeutic target with an unknown activation mechanism. We previously found that the C2-domain of the Saccharomyces cerevisiae Inn1 protein plays an essential but
An electrochemical immunosensor for milk progesterone using a continuous flow system11Editors Selection
Biosensors And Bioelectronics, 16(9), 715-723 (2001)
Effect of diet composition on pregnancy outcome in overnourished rapidly growing adolescent sheep
The British Journal of Nutrition, 96(6), 1060-1068 (2006)
The EMBO journal, 31(9), 2195-2206 (2012-03-22)
Mcm10 is essential for chromosome replication in eukaryotic cells and was previously thought to link the Mcm2-7 DNA helicase at replication forks to DNA polymerase alpha. Here, we show that yeast Mcm10 interacts preferentially with the fraction of the Mcm2-7
EMBO reports, 24(11), e57677-e57677 (2023-10-02)
DONSON is one of 13 genes mutated in a form of primordial microcephalic dwarfism known as Meier-Gorlin syndrome. The other 12 encode components of the CDC45-MCM-GINS helicase, around which the eukaryotic replisome forms, or are factors required for helicase assembly
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