ABScript II One Step RT-qPCR Probe Kit

The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.

ABScript II One Step RT-qPCR Probe Kit is designed for use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed.

For Research Use Only.

Application Note

Additional Materials Required but Not Included:

• RNA templates, primers and probes
• Optical-grade qPCR tubes, plates, sealing films, and aerosol-resistant pipette tips

Compatible Instruments:

*50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne^™/StepOnePlus^™

**50X ROX Reference Dye II
Applied Biosystems 7500/ViiA^™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene^® 3000

NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96^™/ CFX384^™, Roche^® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers^®.

2X One Step RT-qPCR Probe Buffer*
One Step Probe HS Taq
ABScript II RT Enzyme Mix
50X ROX Dye I *
50X ROX Dye II **
RNase-free ddH2O
See datasheet for more information.

Reaction Size
Reaction size is based on a 50 uL reaction volume.

The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:

20RXN: 0.5 mL

100RXN: 2.5 mL

500RXN: 1.25 mL

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Eppendorf is a registered trademark of Eppendorf AG

Mastercycler is a registered trademark of Eppendorf AG

QuantStudio is a trademark of IROA Technologies LLC

Roche is a registered trademark of Roche Molecular Systems, Inc.

Rotor-Gene is a registered trademark of Qiagen GmbH

StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ViiA is a trademark of ThermoFisher