product name
RPMI-1640 培养基, Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture
形式
powder
技术
cell culture | mammalian: suitable
组分
HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
运输
ambient
储存温度
2-8°C
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一般描述
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
应用
RPMI-1640培养基被用于以下应用：
- 用于细胞培养基：补充其他组分的RPMI-1640培养基可用于培养鼠胸腺瘤EL-4细胞
- 不含抗生素的RPMI 1640培养基可用于脾细胞的分枝杆菌感染
- 用于细胞分离和培养
- 作为培养基组分之一，用于淋巴细胞分离和培养、ELISpot酶联免疫斑点测定、离体增殖试验
- 作为培养基，用于gp39基因培养和CD40免疫球蛋白结合试验
- 抗真菌剂制备
数量
按照每升培养基10.4克粉末配制。
重悬
补充2.0 g/L碳酸氢钠。
其他说明
酚红会干扰一些细胞在克隆密度下的生长。该培养基适用于干细胞培养或低密度的细胞培养。也推荐用于体外诊断。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
M E Klepser et al.
Antimicrobial agents and chemotherapy, 41(6), 1392-1395 (1997-06-01)
Time-kill curves were determined for three isolates of Candida albicans tested against fluconazole and amphotericin B at multiples of the MIC. Fluconazole produced fungistatic activity, with concentration-related growth effects observed over a narrow range of concentrations. Amphotericin B exhibited fungicidal
Manuel Cuenca-Estrella et al.
Antimicrobial agents and chemotherapy, 46(11), 3644-3647 (2002-10-18)
A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the
Mathias Lichterfeld et al.
The Journal of experimental medicine, 200(6), 701-712 (2004-09-24)
Virus-specific CD8(+) T cells are associated with declining viremia in acute human immunodeficiency virus (HIV)1 infection, but do not correlate with control of viremia in chronic infection, suggesting a progressive functional defect not measured by interferon gamma assays presently used.
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play
R Roubenoff et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 53(1), M20-M26 (1998-02-19)
To determine the association among aging, inflammation, and cytokine production by peripheral blood mononuclear cells. We examined production of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-1 receptor antagonist (IL-1Ra), and IL-6 in 711 elderly participants in the Framingham
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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