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一般描述
D-核酮糖1,5-二磷酸羧化酶(RUBISCO)占菠菜叶可溶蛋白总量的50%。
以一种557 kDa hexadecamer存在，它由8个分子量约为56 kDa的重链以及8个分子量约为14 kDa的轻链组成。每个分子包含一个镁离子。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
应用
菠菜D-核酮糖1,5-二磷酸羧化酶已用于：
- 作为胃蛋白酶消化研究的测试蛋白
- 检测其作为无害蛋白样品，对人肠上皮细胞系的影响
- 和脱落酸的等温滴定量热法(ITC)和放射性标记结合检测
生化/生理作用
D-核酮糖1,5-二磷酸羧化酶（RUBISCO，核酮糖二磷酸羧化酶）的激活依赖Rubisco激活酶和分子伴侣。它参与植物光吸收事件，可催化核酮糖1,5-二磷酸的羧化和加氧。Rubisco酶的羧化活性受脱落酸抑制。
单位定义
在pH 7.8、25°C条件下，一单位可在每分钟将1.0 μmole的D-RuDP和CO2转化为2.0 μmole的D-3-磷酸甘油酸酯。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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分析证书(COA)
Lot/Batch Number
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