质量水平
形式
powder
技术
cell culture | mammalian: suitable
组分
sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes
运输
ambient
储存温度
2-8°C
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一般描述
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
其他说明
经改良可用于高压灭菌
不含L-谷氨酰胺和碳酸氢钠。
不含L-谷氨酰胺和碳酸氢钠。
数量
按照每升培养基10.3克粉末配制。
重悬
补充0.3 L-谷氨酰胺，2.0 g/L碳酸氢钠。
法律信息
Auto-Mod is a trademark of Sigma-Aldrich Co. LLC
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Zhonghua Liu et al.
mBio, 12(3) (2021-05-20)
Inhibition of fungal growth by Congo red (CR) has been putatively associated with specific binding to β-1,3-glucans, which blocks cell wall polysaccharide synthesis. In this study, we searched for transcription factors (TFs) that regulate the response to CR and interrogated
Leenah Alaalm et al.
Frontiers in cellular and infection microbiology, 11, 637836-637836 (2021-09-14)
The highly conserved, ubiquitous molecular chaperone Hsp90 is a key regulator of cellular proteostasis and environmental stress responses. In human pathogenic fungi, which kill more than 1.6 million patients each year worldwide, Hsp90 governs cellular morphogenesis, drug resistance, and virulence.
Karla Lucía F Alvarez et al.
BMC veterinary research, 16(1), 230-230 (2020-07-08)
In the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells. However
Ryoichi Arita et al.
Diabetes, 58(1), 215-226 (2008-10-09)
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
Fhernanda R Smiderle et al.
BMC complementary and alternative medicine, 11, 58-58 (2011-07-27)
Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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