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Merck
CN

R7131

Sigma-Aldrich

RPMI 1640氨基酸溶液(50×)

Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture

别名:

Amino Acid Supplement

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About This Item

UNSPSC代码:
12352209
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

产品线

BioReagent

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

运输

ambient

储存温度

2-8°C

一般描述

RPMI 1640氨基酸溶液(50×)是氨基酸浓缩液,用于补充以Roswell Park Memorial Institute培养基(RPMI)为基础配方的细胞培养基。

应用

RPMI 1640氨基酸溶液(50×)作为补充剂已用于:
  • 补充M9基础培养基,用于培养细菌菌株SX701和JE116
  • 体外培养哺乳母牛血中性粒细胞
  • 补充非氨基酸Roswell Park Memorial Institute培养基(RPMI),对HeLa细胞进行氨基酸刺激

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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M Garcia et al.
Journal of dairy science, 99(5), 3777-3783 (2016-03-14)
Glutamine is the preferred AA used by polymorphonuclear leukocytes (PMN) during the inflammatory response. However, the effect of other AA on bovine PMN response during inflammation and how this is altered by stage of lactation has not been fully elucidated.
G Ullman et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1611), 20120025-20120025 (2012-12-26)
We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to
D Kazyken et al.
The Journal of biological chemistry, 297(4), 101100-101100 (2021-08-22)
The mechanistic target of rapamycin (mTOR) complex 2 (mTORC2) signaling controls cell metabolism, promotes cell survival, and contributes to tumorigenesis, yet its upstream regulation remains poorly defined. Although considerable evidence supports the prevailing view that amino acids activate mTOR complex
Jakub Wiktor et al.
Nature, 597(7876), 426-429 (2021-09-03)
Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs)1. Initially, the RecBCD complex2 resects the ends of the DSB into 3' single-stranded DNA on which a RecA filament assembles3. Next, the filament locates the homologous repair
Nuno Carinhas et al.
BMC systems biology, 5, 34-34 (2011-03-01)
Stoichiometric models constitute the basic framework for fluxome quantification in the realm of metabolic engineering. A recurrent bottleneck, however, is the establishment of consistent stoichiometric models for the synthesis of recombinant proteins or viruses. Although optimization algorithms for in silico

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