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重组
expressed in E. coli
表单
buffered aqueous glycerol solution
分子量
17.6 kDa
包装
vial of ~30 units
浓度
1,000-4,000 units/mL
运输
dry ice
储存温度
−20°C
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应用
来自大肠杆菌的核糖核酸酶H已被用于评估镁离子的金属生物化学。 核糖核酸酶H还被用于研究HIV-1逆转录酶相关Rnase H活性的选择性抑制剂。
单位定义
一个酶活性单位是指在37°C下，在20分钟内在 3H标记的poly(A) • poly(dT) 中将1.0纳摩尔RNA水解为酸溶性物质所需的酶量。
外形
溶于50%甘油，含有20 mM Tris HCl, pH 7.5, 100 mM KCl，10 mM MgCl2，0.1 mM EDTA，0.1 mM DTT和0.05 mg/ml BSA
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Jessica H Brehm et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 55(5), 737-745 (2012-05-24)
It is not known how often mutations in the connection and ribonuclease H domains of reverse transcriptase (RT) emerge with failure of first-line antiretroviral therapy (ART) in subtype C human immunodeficiency virus type 1 (HIV-1) infection and how these mutations
Virginie Suchaud et al.
Bioorganic & medicinal chemistry letters, 22(12), 3988-3992 (2012-05-23)
We report herein the synthesis of a series of 3-hydroxyquinolin-2(1H)-one derivatives. Esters and amide groups were introduced at position 4 of the basis scaffold and some modulations of the benzenic moiety were performed. Most compounds presented selective inhibitory properties in
H W Huang et al.
European journal of biochemistry, 219(1-2), 253-260 (1994-01-15)
Ribonuclease H (Escherichia coli) contains one strong magnesium-binding site, as determined by metal-titration experiments monitored by high field 1H-NMR and also by direct titration calorimetry. Kinetic and thermodynamic parameters were evaluated by 25Mg-NMR and were as follows: dissociation constant Kd
Mateusz Mendel et al.
Cell, 184(12), 3125-3142 (2021-05-01)
The N6-methyladenosine (m6A) RNA modification is used widely to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (the ortholog of mouse METTL16) deposits an m6A mark on the 3' splice site (AG) of the S-adenosylmethionine
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