生物来源
mouse
质量水平
偶联物
peroxidase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
RabT-50, monoclonal
形式
buffered aqueous solution
种属反应性
rabbit
请勿与下列物质发生反应
human, horse, bovine, cat, rat, chicken, pig, goat, monkey
浓度
2.5-5.0 mg/mL (antibody)
技术
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody
western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
相关类别
一般描述
Monoclonal Anti-Rabbit IgG, Native-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-Rabbit IgG, Native (mouse IgG1 isotype) isolated from hybridoma cell culture of the RabT-50 hybridoma, conjugated to horseradish peroxidase (HRP).
Rabbit immunoglobulins are widely used as primary antibodies in many analytical and research techniques. In immunoprecipitation assays, when target proteins are studied using rabbit primary antibody, the secondary anti-rabbit antibody used for protein detection also recognizes the heavy (50 kDa) and light (25 kDa) chains of the rabbit immunoprecipitating antibody. Consequently, this may conceal the protein of interest, as many proteins appear in the molecular weight range of the immunoglobulin heavy and light chains. The use of monoclonal anti-rabbit IgG-native-peroxidase as the secondary antibody eliminates this hindrance because the antibody is specific for non-reduced rabbit IgG and does not react with reduced rabbit IgG. Moreover, this antibody product does not react with human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs. Thus, this product reduces the background staining that is frequently observed with polyclonal secondary antibodies.
特异性
Monoclonal Anti-Rabbit IgG, Native-Peroxidase reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.
免疫原
Enriched fraction of rabbit IgG light chain.
应用
Monoclonal Anti-Rabbit IgG, Native-Peroxidase antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation
- western blotting
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 0.05% MIT.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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