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安全信息

R1153

Sigma-Aldrich

RNase B 糖蛋白标准品 来源于牛胰腺

≥90% (SDS-PAGE), lyophilized powder

别名:

核糖核酸酶B 来源于牛胰腺, RNase B

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About This Item

CAS号:
9001-99-4
EC 号:
232-646-6
MDL编号:
MFCD00132184
UNSPSC代码:
12352204
NACRES:
NA.32

产品名称

RNase B 糖蛋白标准品 来源于牛胰腺, Proteomics Grade

等级

Proteomics Grade

质量水平

200

方案

≥90% (SDS-PAGE)

表单

lyophilized powder

储存温度

−20°C

SMILES字符串

[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O

InChI

1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)

InChI key

CQOVPNPJLQNMDC-UHFFFAOYSA-N

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相关类别

应用

RNase B是带有PNGase F的优选底物，用于证明使用SDS-PAGE或MALDI-MS进行N联去糖基化。PNGase F的活性通常使用RNase B通过监测去糖基化后在12%凝胶中明显的迁移率变化来测定。蛋白质组学级RNase B在酶消化和随后的纯化后已被用作N-聚糖的来源。它也被用作糖蛋白标准品。

其他说明

牛胰腺核糖核酸酶 B 是一种糖蛋白，仅含有 N-连接聚糖。它是由单个结构域组成的球状蛋白，与作为非糖基化核心形式的核糖核酸酶A（RNase A）在混合物中以较少成分的形式天然存在。RNase B 在 Asn 34 处含有一个糖基化位点，在该位点有 5-9 个甘露糖残基连接到壳二糖核心，即 Man 5-9 GlcNAc 2 。由于 Asn 34 糖基化的异质性，RNase B 以 5 种糖基化变体形式存在，分子量约为 15 kDa。

质量

RNAse B糖蛋白标准品已经过高度纯化，以去除污染的RNaseA。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000397144
0000319070
0000319070
0000397144
SLCR0710

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are there any buffer salts present in product R1153, RNase B Glycoprotein Standard?

    Product R1153, RNase B Glycoprotein Standard, is extensively dialyzed against deionized water prior to lyophilization. It is essentially salt free.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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