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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
secondary antibodies
克隆
R2A-2, monoclonal
包含
15 mM sodium azide
技术
capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:5,000
western blot: suitable
同位素/亚型
IgG1
运输
dry ice
1 of 4
此商品 | MAK266 | MAK270 | MAK215 |
|---|---|---|---|
| detection method fluorometric | detection method colorimetric, fluorometric | detection method fluorometric | detection method fluorometric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| application(s) cosmetics | application(s) - | application(s) cosmetics | application(s) - |
| relevant disease(s) neurological disorders; cardiovascular diseases | relevant disease(s) neurological disorders; cardiovascular diseases | relevant disease(s) - | relevant disease(s) - |
一般描述
Immunoglobulins (IgGs) are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c.
Monoclonal Anti-Rat IgG2a (mouse IgG1 isotype) is derived from the R2A-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG.
特异性
Monoclonal Anti-Rat IgG2a recognizes an epitope located on the heavy chain of rat IgG2a. The antibody detects rat IgG2a derived from normal serum or myeloma proteins, but not the other rat immunoglobulins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with IgG and serum preparations of bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, or sheep.
免疫原
Purified rat IgG
应用
Monoclonal Anti-Rat IgG2a antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- slot blot immunoassay
- immunoblotting
- dot blot
- immunocytochemistry
生化/生理作用
Rat immunoglobulins (IgGs) against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins. Monoclonal Anti-Rat IgG2a antibody is specific for epitope on the heavy chain of rat IgG2a. The antibody localizes the denatured-reduced heavy chain molecule of rat IgG2a (γ2a). The product recognizes rat IgG2a derived from normal serum or myeloma proteins, but does not bind to other rat immunoglobulins. The antibody weakly associates with guinea pig immunoglobulins but does not react with IgG and serum preparations derived from bovine, cat, chicken, dog, goat, horse, human, mouse, pig, rabbit, and sheep.
外形
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
储存及稳定性
For continuous use, store at 0-5 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use,
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
H Bazin et al.
European journal of immunology, 4(1), 44-48 (1974-01-01)
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