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安全信息

QPBCA

Sigma-Aldrich

QuantiPro ™ BCA 检测试剂盒

for 0.5-30 μg/ml protein

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别名:
BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
UNSPSC代码:
12161503
NACRES:
NA.84

质量水平

200

运输

wet ice

储存温度

2-8°C

应用

该试剂盒可在极少量样品中测量极低的蛋白浓度。准确测量蛋白浓度的范围，试管测定为0.5至30 μg/mL；96孔或 384孔平板测定则为1至20 μg/mL。
除了在溶液中测定和定量蛋白质外， QuantiPro™ BCA分析试剂盒还用于其他应用，包括测定与琼脂糖载体共价结合的蛋白质和吸附在多孔板上的蛋白质。 也用于测定各种固体载体上的官能团，如巯基、 N-羟基琥珀酰亚胺基羧酸盐、醛和酰肼。

特点和优势

  • 在广泛的蛋白浓度范围内均能维持高精确度
  • 灵敏度高；蛋白浓度介于0.5 至 30 μg/ml之间时，具有线性响应
  • 稳定的彩色复合物
  • 降低了去垢剂的影响

原理

蛋白质在碱性条件下将Cu2+还原为Cu1+ ，形成二辛可宁酸:1+复合物，该复合物在562 nm处有最大吸光度。

储存及稳定性

室温下储存试剂QA、QB和QC。不使用试剂 QC（4％ 五水硫酸铜（II）溶液12mL），仅将试剂QA和QB混合，置于密闭容器，可在室温下保存一周。如果本试剂盒遇冷或冷藏，则在试剂QA或试剂QB中可能会形成沉淀。要溶解沉淀，请在混合的同时将溶液温度缓慢升至室温。该溶液现在适合使用。

法律信息

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1化学品安全说明书

应用

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