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Merck
CN

PROTTWO

Sigma-Aldrich

ProteoPrep® Universal Extraction Kit

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

应用

本试剂盒含有最新的创新性去垢剂,同时使用了专门配制的试剂,以及为制备两种可直接供二维(2D)电泳使用的亚细胞组分而设计的优化实验方案。
  • 组分1:可溶性/细胞质蛋白
  • 组分2:膜蛋白
通过特殊的还原剂和烷化剂制备出的样本可以在2D凝胶中表现出更高的聚焦度,减少拖尾。ProteoPrep通用提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。

特点和优势

  • 创新的去垢剂制备方法 - 大幅提高了溶解度,可加载更多的蛋白质,提高了2D凝胶中低丰度蛋白的可见性。
  • 两种预混的增溶溶液 - 可以生成两个清洗区分的群落,易于进行2D分析。
  • 预测定的还原剂和烷化剂 - 试剂易于使用,可提供更高的IEF分辨率。
  • 预称重的干混物- 稳定且易于重新配制
  • 方便的包装 - 无废弃物。可按需选择用量。

法律信息

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • C0356Protein Extraction Reagent Type 4 23 mL化学品安全说明书

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5化学品安全说明书

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56化学品安全说明书

警示用语:

Danger

危险分类

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Susanne K Pedersen et al.
Journal of proteome research, 2(3), 303-311 (2003-06-20)
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant proteins identified in most proteomics projects. In yeast, however, low-abundance proteins make up 80% of the predicted proteome, approximately 50% have pl's above pH 8 and
Diliana Dancheva Simeonova et al.
Molecular & cellular proteomics : MCP, 8(1), 122-131 (2008-08-22)
We present here a new approach that enabled the identification of a new protein from a bacterial strain with unknown genomic background using a combination of inverted PCR with degenerate primers derived from N-terminal protein sequences and high resolution peptide
Maria De Angelis et al.
Electrophoresis, 31(13), 2126-2136 (2010-07-02)
Food allergy is recognized as one of the major health concerns. It is estimated that ca. 4% of the population is affected by food allergenic disorders. Food allergies are defined as IgE-mediated hypersensitivity reactions. Foods such as peanuts, tree nuts
W M Freeman et al.
Neuroscience, 159(1), 183-195 (2009-01-13)
Declining cognitive performance is associated with increasing age, even in the absence of overt pathological processes. We and others have reported that declining cognitive performance is associated with age-related changes in brain glucose utilization, long-term potentiation and paired-pulse facilitation, protein
Mustafa R Morsy et al.
Gene, 344, 171-180 (2005-01-20)
Rice (Oryza sativa L.) is sensitive to chilling particularly at early stages of seedling establishment. Two closely related genes (OsLti6a, OsLti6b), which are induced by low temperature during seedling emergence were isolated from a cold tolerant temperate japonica rice cultivar.

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