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一般描述
ProteoPrep®总提取样品试剂盒可从任意类型的细胞或组织中提取所有蛋白质。该试剂盒含有两种在提取应用中广泛使用的试剂及两种增溶能力更强的新试剂，可提取更多蛋白质。该试剂盒还包含用于二硫键还原和烷基化的试剂。因此，我们可以比较四种试剂各自获得的蛋白提取物，并根据您的单独需求进行优化。通过这种专用还原和烷基化试剂制备出的蛋白样品在2D凝胶中可呈现更好的聚集形态、减少条纹。
应用
ProteoPrep®总提取样品试剂盒已用于从各种来源样品提取总蛋白，如表皮干细胞和类风湿关节炎(RA滑膜成纤维细胞。
特点和优势
- 四种预混的增溶溶液 - 可快速溶解。
- 预测定的还原剂和烷化剂 - 试剂易于使用，可提供更高的IEF分辨率。
- 创新的去垢剂制备方法 - 大幅提高了溶解度，可加载更多的蛋白质，提高了2D凝胶中低丰度蛋白的可见性。
储存及稳定性
在未开封容器内，这些试剂至少可保持稳定6个月。
其他说明
ProteoPrep®总提取试剂盒是由Proteome Systems和Sigma的研究科学家合作设计的。
该试剂盒所含试剂足以支持通过全部四种试剂制除至少10份2 ml的样品。对于已经使用某一离液剂提取试剂优化提取方案的研究人员，也可单独选择购买其中的单独试剂。
该试剂盒所含试剂足以支持通过全部四种试剂制除至少10份2 ml的样品。对于已经使用某一离液剂提取试剂优化提取方案的研究人员，也可单独选择购买其中的单独试剂。
法律信息
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
含有大量盐和缓冲液的样品可能不适于采用此试剂盒。这类样品在使用前须进行透析。本产品仅供研发使用，不得用于药物、家庭或其他用途。
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- C0481Protein Extraction Reagent Type 1化学品安全说明书
- C0606Protein Extraction Reagent Type 2化学品安全说明书
- C0731Protein Extraction Reagent Type 3化学品安全说明书
- C0356Protein Extraction Reagent Type 4化学品安全说明书
- A3221Iodoacetamide, Single use vial of 56 mg 5 x 56化学品安全说明书
- T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5化学品安全说明书
警示用语：
Danger
危险分类
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Chi S, et al.
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Tom A Mendum et al.
PloS one, 4(6), e5940-e5940 (2009-06-17)
Despite the introduction of conjugated polysaccharide vaccines for many of the Neisseria meningitidis serogroups, neisserial infections continue to cause septicaemia and meningitis across the world. This is in part due to the difficulties in developing a, cross-protective vaccine that is
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The main cause of steroid-induced necrosis of femoral head (SNFH) is excessive glucocorticoid (GC) intake. The aim of this article was to investigate the role of lncRNA NEAT1 as a molecular sponge to adsorb miR-23b-3p and regulate CYP1A2 in SNFH.
Selim Ceylan et al.
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