storage temp.
2-8°C
Quality Level
Application
ProteoPrep®膜提取试剂盒可从多种类型细胞制备高度富集的膜蛋白溶液。最终制得的蛋白溶液可用于2D凝胶电泳。
Features and Benefits
- 创新的去垢剂制备方法 - 提高了溶解度，可加载更多的蛋白质，提高了2D凝胶中低丰度蛋白的可见性。
- 两种预混的增溶溶液 - 在提取前消除了干扰的非膜蛋白，提高了2D矩阵的整洁度。
- 预测定的还原剂和烷化剂 - 试剂易于使用，可提供更高的IEF分辨率。
General description
ProteoPrep® 膜提取试剂盒试剂采用强力去垢剂来提高2D凝胶的蛋白上样率和分辨率。3和4型Protein Extraction Reagent 针对蛋白质组研究进行了优化。3和4型提取试剂是在等电位聚焦和2D电泳之前进行蛋白提取的最理想产品。 本试剂盒包含进行二硫键还原及烷基化所需的试剂。ProteoPrep® 膜提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
186.8 °F - closed cup
flash_point_c
86 °C - closed cup
Lili Xu et al.
The Journal of general virology, 90(Pt 11), 2695-2703 (2009-07-25)
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has
Sreepriya Nair et al.
Electrophoresis, 32(24), 3621-3629 (2011-11-22)
Performing 2-DE of lipid-rich multilamellar membranes like myelin is a cumbersome task. However, for understanding its molecular organization and changes during diseases, identification of proteins of myelin is essential. Although the 2-D-proteomic approach of myelin has been employed to understand
Haiqing Sheng et al.
Applied and environmental microbiology, 74(16), 5015-5022 (2008-06-17)
Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we
Medicharla Venkata Jagannadham et al.
Journal of proteomics, 75(8), 2488-2499 (2012-03-16)
Antarctic bacteria are adapted to the extremely low temperature. The transcriptional and translational machineries of these bacteria are adapted to the sub-zero degrees of temperature. Studies directed towards identifying the changes in the protein profiles during changes in the growth
Neal X Chen et al.
American journal of physiology. Renal physiology, 292(2), F599-F606 (2006-09-14)
Fetuin-A is a known inhibitor of vascular calcification in vitro. In arteries with calcification, there is increased immunostaining for fetuin-A. However, vascular smooth muscle cells (VSMC) do not synthesize fetuin-A, suggesting fetuin-A may be endocytosed to exert its inhibitory effects.
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