推荐产品
一般描述
ProteoPrep® 膜提取试剂盒试剂采用强力去垢剂来提高2D凝胶的蛋白上样率和分辨率。3和4型Protein Extraction Reagent 针对蛋白质组研究进行了优化。3和4型提取试剂是在等电位聚焦和2D电泳之前进行蛋白提取的最理想产品。 本试剂盒包含进行二硫键还原及烷基化所需的试剂。ProteoPrep® 膜提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。
应用
ProteoPrep®膜提取试剂盒可从多种类型细胞制备高度富集的膜蛋白溶液。最终制得的蛋白溶液可用于2D凝胶电泳。
特点和优势
- 创新的去垢剂制备方法 - 提高了溶解度，可加载更多的蛋白质，提高了2D凝胶中低丰度蛋白的可见性。
- 两种预混的增溶溶液 - 在提取前消除了干扰的非膜蛋白，提高了2D矩阵的整洁度。
- 预测定的还原剂和烷化剂 - 试剂易于使用，可提供更高的IEF分辨率。
法律信息
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险分类
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
闪点(°F)
186.8 °F - closed cup
闪点(°C)
86 °C - closed cup
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Sreepriya Nair et al.
Electrophoresis, 32(24), 3621-3629 (2011-11-22)
Performing 2-DE of lipid-rich multilamellar membranes like myelin is a cumbersome task. However, for understanding its molecular organization and changes during diseases, identification of proteins of myelin is essential. Although the 2-D-proteomic approach of myelin has been employed to understand
Lili Xu et al.
The Journal of general virology, 90(Pt 11), 2695-2703 (2009-07-25)
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has
Fang Chen et al.
Proteomics, 7(9), 1529-1539 (2007-04-05)
Plant plasma membrane (PM) proteins play important roles in signal transduction during defense response to an attacking pathogen. By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker, we conducted
Carla Cacciotto et al.
BMC microbiology, 10, 225-225 (2010-08-27)
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial
Ji Youn Lim et al.
Applied and environmental microbiology, 73(7), 2037-2047 (2007-02-06)
Escherichia coli O157:H7 causes hemorrhagic colitis and hemolytic-uremic syndrome in humans, and its major reservoir is healthy cattle. An F-like 92-kb plasmid, pO157, is found in most E. coli O157:H7 clinical isolates, and pO157 shares sequence similarities with plasmids present
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