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Merck
CN
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文件

PROTMEM

Millipore

ProteoPrep® 膜提取试剂盒

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UNSPSC代码:
12352200
NACRES:
NA.32

一般描述

ProteoPrep® 膜提取试剂盒试剂采用强力去垢剂来提高2D凝胶的蛋白上样率和分辨率。3和4型Protein Extraction Reagent 针对蛋白质组研究进行了优化。3和4型提取试剂是在等电位聚焦和2D电泳之前进行蛋白提取的最理想产品。 本试剂盒包含进行二硫键还原及烷基化所需的试剂。ProteoPrep® 膜提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。

应用

ProteoPrep®膜提取试剂盒可从多种类型细胞制备高度富集的膜蛋白溶液。最终制得的蛋白溶液可用于2D凝胶电泳。

特点和优势

  • 创新的去垢剂制备方法 - 提高了溶解度，可加载更多的蛋白质，提高了2D凝胶中低丰度蛋白的可见性。
  • 两种预混的增溶溶液 - 在提取前消除了干扰的非膜蛋白，提高了2D矩阵的整洁度。
  • 预测定的还原剂和烷化剂 - 试剂易于使用，可提供更高的IEF分辨率。

法律信息

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5化学品安全说明书
  • C0356Protein Extraction Reagent Type 4 23 mL化学品安全说明书
  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56化学品安全说明书

警示用语：

Danger

危险分类

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

186.8 °F

闪点(°C)

86 °C

分析证书(COA)

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Medicharla Venkata Jagannadham et al.
Journal of proteomics, 75(8), 2488-2499 (2012-03-16)
Antarctic bacteria are adapted to the extremely low temperature. The transcriptional and translational machineries of these bacteria are adapted to the sub-zero degrees of temperature. Studies directed towards identifying the changes in the protein profiles during changes in the growth
Ji Youn Lim et al.
Applied and environmental microbiology, 73(7), 2037-2047 (2007-02-06)
Escherichia coli O157:H7 causes hemorrhagic colitis and hemolytic-uremic syndrome in humans, and its major reservoir is healthy cattle. An F-like 92-kb plasmid, pO157, is found in most E. coli O157:H7 clinical isolates, and pO157 shares sequence similarities with plasmids present
Neal X Chen et al.
American journal of physiology. Renal physiology, 292(2), F599-F606 (2006-09-14)
Fetuin-A is a known inhibitor of vascular calcification in vitro. In arteries with calcification, there is increased immunostaining for fetuin-A. However, vascular smooth muscle cells (VSMC) do not synthesize fetuin-A, suggesting fetuin-A may be endocytosed to exert its inhibitory effects.
Haiqing Sheng et al.
Applied and environmental microbiology, 74(16), 5015-5022 (2008-06-17)
Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we
Fang Chen et al.
Proteomics, 7(9), 1529-1539 (2007-04-05)
Plant plasma membrane (PM) proteins play important roles in signal transduction during defense response to an attacking pathogen. By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker, we conducted
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