Analysis Note
已测试过其在全部4种蛋白酶水解类别中的检测效果（丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶）。
Application
检测限：最大50ng胰蛋白酶
蛋白酶荧光检测试剂盒已用于：
- 通过基于酪蛋白的蛋白酶测定，检测蛋白水解活性
- 检测真菌菌株的细胞外蛋白水解活性
- 定量线粒体蛋白酶活性
- 测定土壤溶液中的蛋白酶生成
Preparation Note
提供了足以用于200次一毫升反应体系的试剂。
三氯乙酸（TCA）组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。
三氯乙酸（TCA）组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
Joseph S Rom et al.
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Staphylococcus aureus causes acute and chronic forms of infection, the latter often associated with formation of a biofilm. It has previously been demonstrated that mutation of atl, codY, rot, sarA, and sigB limits biofilm formation in the USA300 strain LAC
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Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic
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Volonte D, et al.
Aging (Albany. NY.), 8(10), 2355-2355 (2016)
Contrasting effects of increased carbon input on boreal SOM decomposition with and without presence of living root system of Pinus sylvestris L.
Linden, A., et al.
Plant and Soil, 377, 145-158 (2014)
Martin H Kunzmann et al.
Molecular bioSystems, 8(11), 3061-3067 (2012-09-20)
α-Alkylidene-γ-butyrolactones are quite common in nature and exhibit a broad spectrum of biological activities. We therefore synthesized a small library of xanthatine inspired α-alkylidene-γ-butyrolactones to screen non-pathogenic and uropathogenic E. coli strains by activity based protein profiling (ABPP). The identified
商品
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
实验方案
Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.
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