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Merck
CN

PF0100

Sigma-Aldrich

蛋白酶检测试剂盒(荧光法)

high sensitivity assay

别名:

蛋白酶活性分析试剂盒

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.26

质量水平

运输

dry ice

储存温度

−20°C

应用

蛋白酶荧光检测试剂盒已用于:
  • 通过基于酪蛋白的蛋白酶测定,检测蛋白水解活性
  • 检测真菌菌株的细胞外蛋白水解活性
  • 定量线粒体蛋白酶活性
  • 测定土壤溶液中的蛋白酶生成

适用性

检测限:最大50ng胰蛋白酶

数量

提供了足以用于200次一毫升反应体系的试剂。
提供了足以用于200次一毫升反应体系的试剂。

三氯乙酸(TCA)组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。

分析说明

已测试过其在全部4种蛋白酶水解类别中的检测效果(丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶)。

仅试剂盒组分

产品编号
说明

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

警示用语:

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Veronica Tjomsland et al.
European journal of immunology, 43(6), 1470-1483 (2013-03-26)
Induction of optimal HIV-1-specific T-cell responses, which can contribute to controlling viral infection in vivo, depends on antigen processing and presentation processes occurring in DCs. Opsonization can influence the routing of antigen processing and pathways used for presentation. We studied
Alejandro Soto-Gutiérrez et al.
Nature protocols, 2(2), 347-356 (2007-04-05)
This protocol describes a co-culture system for the in vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells. Differentiation involves four steps: (i) formation of embryoid bodies (EB), (ii) induction of definitive endoderm from 2-d-old EBs, (iii) induction of
Dramatic increase in stability and longevity of enzymes attached to monodispersive iron nanoparticles.
Sharma A, et al.
IEEE Transactions on Magnetics, 43, 2418-2420 (2007)
Stimulation of soil organic nitrogen pool: The effect of plant and soil organic matter degrading enzymes
Kieloaho AJ, et al.
Soil Biology and Biochemistry, 96(10), 97-106 (2016)
Céline Adam et al.
PloS one, 7(5), e38138-e38138 (2012-06-14)
Mitochondria have their own ATP-dependent proteases that maintain the functional state of the organelle. All multicellular eukaryotes, including filamentous fungi, possess the same set of mitochondrial proteases, unlike in unicellular yeasts, where ClpXP, one of the two matricial proteases, is

商品

Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.

实验方案

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

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