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Merck
CN

PF0100

Sigma-Aldrich

蛋白酶检测试剂盒(荧光法)

high sensitivity assay

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别名:
Protease Assay Kit
UNSPSC代码:
12352202
NACRES:
NA.26

质量水平

运输

dry ice

储存温度

−20°C

应用

蛋白酶荧光检测试剂盒已用于:
  • 通过基于酪蛋白的蛋白酶测定,检测蛋白水解活性
  • 检测真菌菌株的细胞外蛋白水解活性
  • 定量线粒体蛋白酶活性
  • 测定土壤溶液中的蛋白酶生成

适用性

检测限:最大50ng胰蛋白酶

数量

提供了足以用于200次一毫升反应体系的试剂。
提供了足以用于200次一毫升反应体系的试剂。

三氯乙酸(TCA)组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。

分析说明

已测试过其在全部4种蛋白酶水解类别中的检测效果(丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶)。

仅试剂盒组分

产品编号
说明

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

警示用语:

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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实验方案

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

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