一般描述
分离所得的过氧化物酶体可用于研究脂质β-氧化、氨基酸代谢及醚链连接的甘油糖脂和胆汁酸的生物合成。
应用
过氧化物酶体分离试剂盒提供了使用碘克沙醇[OptiPrep™]的密度梯度离心法从动物组织和细胞质分离高度纯化的过氧化物酶体所需的一切试剂及详细实验方案。该试剂盒还可用于通过大鼠肝脏、大鼠肾脏、兔肝脏及HEK293和HepG2细胞制备过氧化物酶体。
特点和优势
- 专为研究规模应用而配制的提取试剂 - 有助于节省时间，最大限度减少浪费
- 可获得完整的功能性细胞器 - 所得的过氧化物酶体适于功能性研究、代谢检测、蛋白谱分析和疾病状态分析
- 兼容结构验证产品 - 可采用随附的细胞色素c还原酶检测试剂盒轻松进行完整性验证（货号：CY0100）
其他说明
收到试剂盒后，蛋白酶抑制剂混合物（产品代码 P 8340）应储存在 –20°C 条件下，OptiPrep 密度梯度培养基（产品代码 O3028）应储存在室温下。
法律信息
OptiPrep is a trademark of Serumwerk Bernburg AG
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
储存分类代码
8A - Combustible corrosive hazardous materials
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
法规信息
常规特殊物品
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
K Burdett et al.
The Journal of biological chemistry, 266(19), 12201-12206 (1991-07-05)
Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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