P9529
3′,5′-环核苷酸磷酸二酯酶（特异性） 来源于牛大脑
lyophilized powder
别名:
3′,5′-环核苷酸 5′-核苷酸水解酶 来源于牛大脑, PDE
biological source
bovine brain
form
lyophilized powder
specific activity
1-5 units/mg protein (without added calmodulin)
mol wt
~60 kDa
composition
Protein, 4.0-6.0% Lowry
UniProt accession no.
foreign activity
2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%, 5′-ATPase ≤0.2%, inorganic pyrophosphatase ≤0.8%
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
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General description
Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.
Application
Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.
Biochem/physiol Actions
水解环单磷酸核苷酸（如cAMP 和 cGMP）的 3′,5′-磷酸二酯，生成相应的 5′-单磷酸核苷酸。
Packaging
根据活化单位确定包装尺寸
Physical form
含 Tris-HCl 缓冲盐和乳糖的冻干粉
Preparation Note
已耗尽钙调蛋白的亲和纯化制剂。
用 50% 甘油复原。在 -20°C 下储存，酶的总活化单位可保持至少 5 天的恒定状态。但是，钙调蛋白不足时的活性可增加至 200%。如果在 4°C 下储存，激活时的活性和钙调蛋白不足时的活性都将在 24 小时内下降约 30%。
Analysis Note
由 Warburg-Christian 法测定的蛋白质
Other Notes
在 pH 7.5 和 30°C 下，一单位本品每分钟可将 1.0μmol 3′,5′-cAMP 水解为 5′-AMP。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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P D Calvert et al.
The Journal of general physiology, 111(1), 39-51 (1998-02-14)
Light adaptation in vertebrate photoreceptors is thought to be mediated through a number of biochemical feedback reactions that reduce the sensitivity of the photoreceptor and accelerate the kinetics of the photoresponse. Ca2+ plays a major role in this process by
M D Houslay
Seminars in cell & developmental biology, 9(2), 161-167 (1998-05-26)
Cyclic AMP has provided the paradigm for the second messenger concept. Recent evidence has identified a complex array of isoforms of the enzymes involved in generation, destroying and transmitting the effects of this second messenger. These proteins provide a sophisticated
Drummond, G.I. And Yamamoto, M. et al.
The Enzymes (1971)
Vibrio vulnificus Biotype 3 Multifunctional Autoprocessing RTX Toxin Is an Adenylate Cyclase Toxin Essential for Virulence in Mice
Kevin J. Ziolo
Infection (Munich) (2014)
Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation
Kok-Long Ang Ferenc A. Antoni
Journal of Neurochemistry (2002)
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