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生物来源
bovine brain
表单
lyophilized powder
比活
1-5 units/mg protein (without added calmodulin)
分子量
~60 kDa
组成
Protein, 4.0-6.0% Lowry
UniProt登记号
异质活性
2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%
5′-ATPase ≤0.2%
inorganic pyrophosphatase ≤0.8%
储存温度
−20°C
基因信息
cow ... PDE1A(281969)
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一般描述
Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.
应用
Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.
生化/生理作用
水解环单磷酸核苷酸（如cAMP 和 cGMP）的 3′,5′-磷酸二酯，生成相应的 5′-单磷酸核苷酸。
包装
根据活化单位确定包装尺寸
单位定义
在 pH 7.5 和 30°C 下，一单位本品每分钟可将 1.0μmol 3′,5′-cAMP 水解为 5′-AMP。
外形
含 Tris-HCl 缓冲盐和乳糖的冻干粉
制备说明
已耗尽钙调蛋白的亲和纯化制剂。
重悬
用 50% 甘油复原。在 -20°C 下储存，酶的总活化单位可保持至少 5 天的恒定状态。但是，钙调蛋白不足时的活性可增加至 200%。如果在 4°C 下储存，激活时的活性和钙调蛋白不足时的活性都将在 24 小时内下降约 30%。
分析说明
由 Warburg-Christian 法测定的蛋白质
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Drummond, G.I. And Yamamoto, M. et al.
The Enzymes (1971)
Vibrio vulnificus Biotype 3 Multifunctional Autoprocessing RTX Toxin Is an Adenylate Cyclase Toxin Essential for Virulence in Mice
Kevin J. Ziolo
Infection (Munich) (2014)
Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation
Kok-Long Ang Ferenc A. Antoni
Journal of Neurochemistry (2002)
Calmodulin-dependent cyclic nucleotide phosphodiesterase (PDE1) splice variants from bovine cardiac muscle.
Das SB
Gene, 396(2), 283-292 (2007)
M D Houslay
Seminars in cell & developmental biology, 9(2), 161-167 (1998-05-26)
Cyclic AMP has provided the paradigm for the second messenger concept. Recent evidence has identified a complex array of isoforms of the enzymes involved in generation, destroying and transmitting the effects of this second messenger. These proteins provide a sophisticated
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