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Merck
CN

P8999

Sigma-Aldrich

抗p53抗体,小鼠单克隆 小鼠抗

clone DO-7, purified from hybridoma cell culture

别名:

抗 TRP53, 抗 转化相关蛋白 53

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

DO-7, monoclonal

表单

buffered aqueous solution

分子量

antigen ~53 kDa

种属反应性

human

浓度

~2 mg/mL

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

同位素/亚型

IgG2b

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... TP53(7157)

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一般描述

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

应用

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

生化/生理作用

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.
p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas
Ishii HH, et al.
Journal of Clinical Pathology, 57(12), 1306-1311 (2004)
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53
Chehab NH, et al.
Genes & Development, 14(3), 278-288 (2000)
New Insights into p53 Signaling and Cancer Cell Response to DNA Damage: Implications for Cancer Therapy.
Mirzayans, R., et al.
Journal of Biomedicine and Biotechnology, doi: 10-doi: 10 (2012)
DNA damage-induced activation of p53 by the checkpoint kinase Chk2
Hirao A, et al.
Science (New York, N.Y.), 287(5459), 1824-1827 (2000)
Predictors of progression in Barrett's esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression
Reid BJ, et al.
The American Journal of Gastroenterology, 96(10), 2839-2839 (2001)

商品

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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