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安全信息

P8651

Sigma-Aldrich

蛋白A-过氧物酶 来源于金黄色酿脓葡萄球菌/辣根

lyophilized powder

别名:

过氧物酶-蛋白A

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About This Item

MDL编号:
MFCD00132102
UNSPSC代码:
12352203
NACRES:
NA.46

偶联物

peroxidase conjugate

质量水平

100
200

表单

lyophilized powder

技术

direct ELISA: 1:80,000 using human IgG

储存温度

−20°C

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一般描述

蛋白A是金黄色葡萄球菌的细胞壁成分，分子量为56kDa。它具有五个结合结构域，可与Fc或Fab片段的免疫球蛋白重链发生相互作用。蛋白A可激活补体系统、干扰素产生、超敏反应和细胞介导的细胞毒性作用。辣根过氧化物酶是一种含血红素的酶。 在过氧化氢存在下，该酶催化鲁米诺的氧化。

特异性

仅与大多数哺乳动物的IgG结合，除了大鼠、山羊、绵羊。

应用

来自金黄色葡萄球菌/辣根的蛋白A-过氧化物酶已用于酶联免疫吸附测定（ELISA）。

包装

包装规格基于蛋白含量。

外形

冻干粉末，含有柠檬酸钠缓冲盐。通过A205测定蛋白质。

制备说明

通过改良O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978)中所报道的有利于低分子量偶联物的实验方法，使用VI型过氧化物酶标记细胞外蛋白A。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000329693
0000329693
SLCP2555
SLCH3263
SLCG0737

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I prepare a stock solution of Product P8651, Protein A Peroxidase?

    A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.

  4. What is the solution stability of Product P8651, Protein A Peroxidase?

    The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.

  5. How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?

    For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.

  6. I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?

    P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Neosporosis and besnoitiosis, caused by cyst-forming protozoa Neospora caninum and Besnoitia besnoiti, respectively, are parasitic infestations of livestock in Israel. These parasites cause significant economic losses in cattle due to reproductive and productive disorders. Both parasites have been detected in
Katharina Mayer et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Within-host adaptation is a typical feature of chronic, persistent Staphylococcus aureus infections. Research projects addressing adaptive changes due to bacterial in-host evolution increase our understanding of the pathogen's strategies to survive and persist for a long time in various hosts
J Jelfs et al.
Epidemiology and infection, 120(3), 263-270 (1998-08-06)
Fourteen cases of meningococcal disease (MD) occurred in August September 1996 in western Sydney, Australia. Seven of the 10 young adults affected had a direct or indirect link with a local nightclub. Ten of 11 systemic meningococcal isolates had the
Staphylococcal protein A consists of five IgG-binding domains
Tomas MOKS
European Journal of Biochemistry (1986)
P Eliades et al.
Journal of immunological methods, 218(1-2), 123-132 (1998-11-18)
We have developed a dot immunobinding assay (CM) for the serodiagnosis of the human hydatidosis. The assay employs Samaron blue colloidal dye particles conjugated to hydatid antigen (HA) or protein A, both of which serve as visualizing agents. The test
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实验方案

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

过氧化物酶的酶学测定（EC 1.11.1.7）

该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。

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