product name
聚-D-赖氨酸 氢溴酸盐, mol wt 30,000-70,000
形式
solid
质量水平
分子量
30,000-70,000
技术
cell culture | mammalian: suitable
颜色
white to off-white
储存温度
−20°C
SMILES字符串
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
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应用
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
生化/生理作用
多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。
组分
聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意
无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
制备说明
为了除去HBr，可将该产品溶解在中性缓冲液中并透析以除去盐。 一般来说，要将此产品用作附着因子，可将50 mL无菌组织培养级水加入至5 mg聚赖氨酸中，并按每25cm2 1 mL的比例将溶液无菌涂抹在表面上。 5分钟后，通过抽吸除去溶液并彻底冲洗表面。 在引入细胞和培养基之前让其干燥两小时。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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