一般描述
Monoclonal Anti-p53 (mouse IgG1 isotype) is derived from the hybridoma DO-2 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.
免疫原
recombinant human wild type p53. The antibody epitope resides between amino acids 10-16 of human p53.
应用
Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for DNase I footprint, immunoprecipitation and western blotting.
生化/生理作用
Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.
p53 is phosphorylated at multiple sites by several protein kinases in response to DNA damage. It is essential for the maintenance of the non-tumorigenic phenotype of cells. Phosphorylation of p53 at Ser15 by ataxia telangiectasia mutate (ATM), ataxia telangiectasia and Rad3-related protein (ATR), and DNA-dependent protein kinase (DNA-PK) leads to a reduced interaction with its negative regulator MDM2 and accumulation of p53 protein. Checkpoint kinase 2 (Chk2) and Chk1 can phosphorylate p53 at Ser20, which enhances its activity, tetramerization, and stability. Elevation of p53 protein induces the transcriptional activation of multiple genes, including cyclin-dependent kinase inhibitor 1 (p21waf1).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
