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主要文件

安全信息

P7640

Sigma-Aldrich

碱性磷酸酶 来源于牛肠粘膜

lyophilized powder, ≥10 DEA units/mg solid

别名:

Orthophosphoric-monoester phosphohydrolase (alkaline optimum), 碱性磷酸酶

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1 G
¥344.58
5 G
¥3,961.20

¥344.58

目录价¥1,148.62节省 70%

国内现货,预计发货时间2025年4月25日详情


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1 G
¥344.58
5 G
¥3,961.20

About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
eCl@ss:
42010105
NACRES:
NA.54

¥344.58

目录价¥1,148.62节省 70%

国内现货,预计发货时间2025年4月25日详情


获取大包装报价

生物来源

bovine intestinal mucosa

质量水平

表单

lyophilized powder

比活

≥10 DEA units/mg solid

分子量

dimer ~160 kDa

运输

wet ice

储存温度

−20°C

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此商品
SAB4501593WH0003708M1AB9074
Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

serum

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

rabbit

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

Gene Information

human ... ITPR1(3708)

Gene Information

human ... ITPR1(3708)

Gene Information

human ... ITPR1(3708)

Gene Information

human ... ITPR2(3709)

species reactivity

rat

species reactivity

rat, human, mouse

species reactivity

human

species reactivity

human, mouse

应用

推荐用于抗体和蛋白质缀合的高比活性等级。
碱性磷酸酶可用于与抗体和其他蛋白质缀合,以用于ELISA、Western印迹和组织化学检测。 其常用于蛋白质和核酸的去磷酸化。 当需要高灵敏度时,它可用于蛋白质标记。 碱性磷酸酶也可用于使DNA或RNA的5′-末端去磷酸化以防止其发生自身连接。在用碱性磷酸酶去磷酸化后,DNA或RNA也可用放射性标记的磷酸盐(通过T4多核苷酸激酶)进行标记。 产品P7640已被用于研究单克隆碱性磷酸酶-抗碱性磷酸酶(APAAP)复合物[1]
碱性磷酸酶可用于酪蛋白和其他蛋白质的去磷酸化。碱性磷酸酶还可用于 DNA 或 RNA 的 5′-末端的去磷酸化,以防止自我连接。经碱性磷酸酶的去磷酸化作用后,DNA 或 RNA 还可被放射性标记磷酸盐标记(通过 T4 多核苷酸激酶)。

生化/生理作用

来自牛肠粘膜的碱性磷酸酶在7.5-9.5的pH范围内最稳定。该酶对醇、胺、焦磷酸盐和酚的磷酸酯具有广泛的特异性,并且其需要锌和镁或钙二价离子来进行活化。
该酶对醇类、胺类、焦磷酸盐以及酚类的磷酸酯具有广泛的特异性。常用于蛋白质和核酸的去磷酸化。

物理属性

牛肠碱性磷酸酶为二聚膜衍生糖蛋白。至少存在三种同工酶,每个单体通常拥有两个 N-连接多糖和一个或多个 O-连接多糖。2 该酶需要锌以及镁或钙二价离子才能显示活性。
牛肠道碱性磷酸酶是一种二聚化的膜衍生糖蛋白。其存在至少三种异构体,每个单体通常具有两个N-连接的和一个或多个O-连接的聚糖。

单位定义

在pH 9.8,37℃条件下,一个DEA单位可每分钟水解1 μmole的4-硝基苯基磷酸酯。(一个甘氨酸单位相当于~3个DEA单位)

分析说明

包装规格取决于 DEA 单位

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
  • 技术规格说明书

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    Mamoni Dash et al.
    Carbohydrate polymers, 182, 254-264 (2017-12-28)
    Polyelectrolyte complexes (PEC) of chitosan and ulvan were fabricated to study alkaline phosphatase (ALP) mediated formation of apatitic minerals. Scaffolds of the PEC were subjected to ALP and successful mineral formation was studied using SEM, Raman and XRD techniques. Investigation
    A Hohmann et al.
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 36(2), 137-143 (1988-02-01)
    A mouse monoclonal antibody, FMC55 (an IgG1), to alkaline phosphatase was prepared and evaluated in immunostaining. Clones producing antibody to alkaline phosphatase were selected using a micro-ELISA which identified antibodies forming active soluble complexes (APAAP) with the enzyme. Conditions that
    Simultaneous flow-injection determination of fluoride, monofluorophosphate and orthophosphate ions using alkaline phosphatase immobilized on a cellulose nitrate membrane and an open-circulation approach.
    Tzanavaras PD and Themelis DG
    Analytica Chimica Acta, 467 (1-2), 83-89 (2002)
    Stefanie Riedel et al.
    Journal of functional biomaterials, 12(4) (2021-10-27)
    Biological hydrogels are highly promising materials for bone tissue engineering (BTE) due to their high biocompatibility and biomimetic characteristics. However, for advanced and customized BTE, precise tools for material stabilization and tuning material properties are desired while optimal mineralisation must
    Some properties of alkaline phosphatase of cow's milk and calf intestinal mucosa.
    R K MORTON
    The Biochemical journal, 60(4), 573-582 (1955-08-01)

    实验方案

    Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

    碱性磷酸酶的酶法测定,二乙醇胺法(EC 3.1.3.1)

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