相关类别
应用
该产品已用于植物组织中焦磷酸盐含量的测定。通过监测马铃薯块茎提取物中的NADH氧化，它也已用作焦磷酸盐含量的快速常规检查。
生化/生理作用
焦磷酸盐检测试剂盒将焦磷酸盐的产生与NADH的消耗偶联在一起。
包装
包括技术公告。
组分
组成浓度
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
质量
一种包含果糖-6-磷酸激酶（焦磷酸依赖性）、醛缩酶、磷酸丙糖异构酶和α-甘油磷酸脱氢酶的偶联酶系统。
原理
每消耗一摩尔焦磷酸盐，两摩尔NADH被氧化为NAD+。在340 nm处通过分光光度法监测反应。
该试剂可用于检测10-100 nmole的焦磷酸盐。
该试剂可用于检测10-100 nmole的焦磷酸盐。
制备说明
将一瓶焦磷酸盐试剂的内容物溶于4.0 mL水中。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
J A Kern et al.
Biotechnology progress, 13(6), 747-756 (1997-12-31)
Solution equilibrium analysis of in vitro RNA transcription has been applied to examine changes in pH, free magnesium concentration, and concentrations of all chemical ionization species as a transcription reaction proceeds. With this method, the progress of a transcription reaction
Andras Szollosi et al.
The Journal of general physiology, 137(6), 549-562 (2011-05-18)
Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel belonging to the adenosine triphosphate (ATP)-binding cassette (ABC) superfamily. ABC proteins share a common molecular mechanism that couples ATP binding and hydrolysis at two nucleotide-binding domains (NBDs) to diverse functions.
A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation.
W E O'Brien
Analytical biochemistry, 76(2), 423-430 (1976-12-01)
Erick Strauss et al.
The Journal of biological chemistry, 277(50), 48205-48209 (2002-10-10)
Pantothenic acid (vitamin B(5)) is the natural precursor of coenzyme A (CoA), an essential cofactor in all organisms. The pantothenic acid antimetabolite N-pentylpantothenamide inhibits the growth of Escherichia coli with a minimum inhibitory concentration of 2 microm. In this study
William Swinehart et al.
RNA (New York, N.Y.), 26(9), 1094-1103 (2020-05-10)
N6-threonylcarbamoyl adenosine (t6A) is a nucleoside modification found in all kingdoms of life at position 37 of tRNAs decoding ANN codons, which functions in part to restrict translation initiation to AUG and suppress frameshifting at tandem ANN codons. In Bacteria
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