P6874

Anti- p53 抗体，小鼠单克隆

Name: Anti- p53 抗体，小鼠单克隆
Brand: SIGMA

clone DO-1, purified from hybridoma cell culture

别名:

Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53

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关于此项目

NACRES:

NA.41

UNSPSC Code:

12352203

主要文件

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产品名称

Anti- p53 抗体，小鼠单克隆, clone DO-1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells

isotype

IgG2a

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... TP53(7157)

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Application

小鼠单克隆抗p53抗体已被用于：

半干转免疫印迹（semi-dry western blotting）
免疫印迹
免疫荧光
酶联免疫吸附测定（ELISA）
免疫细胞化学
免疫组织化学
免疫沉淀
流式细胞术
染色质免疫沉淀（ChIP）分析

Biochem/physiol Actions

p53肿瘤抑制蛋白在细胞对DNA损伤和其他基因组畸变的反应中很重要。p53蛋白的升高会诱导包括p21^awf1在内的多个基因的转录激活。研究表明野生型p53是一种序列特异性转录因子，可以直接与各种细胞和病毒蛋白相互作用。完整的p53功能对于维持细胞的非致瘤表型必不可少。p53在抑制癌症的发展、细胞周期调节和CAK激酶活性的调节中起着关键作用。

小鼠抗（p53）抗体可与人类（p53）发生特异性反应。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

单克隆抗p53（小鼠IgG2a同种型）来源于小鼠骨髓瘤细胞（SP2细胞）和重组人野生型p53免疫的BALB/c小鼠脾细胞融合产生的DO-1杂交瘤。 p53基因位于人染色体17p上。该基因编码在正常组织中表达的肿瘤抑制蛋白。

Immunogen

重组人野生型 p53。表位位于人 p53 的 20-25 个氨基酸之间。

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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分析证书(COA)

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A long noncoding RNA distributed in both nucleus and cytoplasm operates in the PYCARD-regulated apoptosis by coordinating the epigenetic and translational regulation.

Hui Miao et al.

PLoS genetics, 15(5), e1008144-e1008144 (2019-05-16)

Long noncoding RNAs (lncRNAs) participate in various biological processes such as apoptosis. The function of lncRNAs is closely correlated with their localization within the cell. While regulatory potential of many lncRNAs has been revealed at specific subcellular location, the biological

Silencing of ribosomal protein S9 elicits a multitude of cellular responses inhibiting the growth of cancer cells subsequent to p53 activation.

Mikael S Lindström et al.

PloS one, 5(3), e9578-e9578 (2010-03-12)

Disruption of the nucleolus often leads to activation of the p53 tumor suppressor pathway through inhibition of MDM2 that is mediated by a limited set of ribosomal proteins including RPL11 and RPL5. The effects of ribosomal protein loss in cultured

P21Cip1/WAF1 downregulation is required for efficient PCNA ubiquitination after UV irradiation.

G Soria et al.

Oncogene, 25(20), 2829-2838 (2006-01-13)

p21(Cip1/WAF1) is a known inhibitor of the short-gap filling activity of proliferating cell nuclear antigen (PCNA) during DNA repair. In agreement, p21 degradation after UV irradiation promotes PCNA-dependent repair. Recent reports have identified ubiquitination of PCNA as a relevant feature

Knockdown of hnRNPK leads to increased DNA damage after irradiation and reduces survival of tumor cells

Wiesmann N, et al.

Carcinogenesis, 38(3), 321-328 (2017)

ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions.

Samantha J McDonnell et al.

Cell death discovery, 5, 132-132 (2019-09-12)

Specific molecular interactions that underpin the switch between ER stress-triggered autophagy-mediated cellular repair and cellular death by apoptosis are not characterized. This study reports the unexpected interaction elicited by ER stress between the plasma membrane (PM)-localized apoptosis effector PERP and

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Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

癌症干细胞：癌症治疗的靶标

培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。

Anti- p53 抗体，小鼠单克隆

clone DO-1, purified from hybridoma cell culture

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

conjugate

antibody form

antibody product type

clone

form

mol wt

species reactivity

concentration

technique(s)

isotype

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Quality Level

Gene Information

相关类别

说明

Application

Biochem/physiol Actions

Disclaimer

General description

Immunogen

Physical form

安全信息

存储类别

wgk

flash_point_f

flash_point_c

ppe

法规信息

文件

分析证书(COA)

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