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产品名称
聚-D-赖氨酸 氢溴酸盐, mol wt 4,000-15,000
表单
powder or solid
质量水平
分子量
4,000-15,000
技术
cell culture | mammalian: suitable
颜色
white to light yellow
储存温度
−20°C
SMILES字符串
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
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相关类别
应用
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
生化/生理作用
多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。
组分
聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意
无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
制备说明
本品的分子量为4,000-15,000。为除去HBr，将本品溶解在中性缓冲液中，并透析除盐。通常，将本品用作黏附因子，将50 mL无菌组织培养级水加至5 mg聚赖氨酸中，并在每25 cm2表面上用1 mL溶液无菌涂抹。5分钟后，吸去溶液并彻底清洗表面。先干燥两小时，再加入细胞和培养基。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Munehiro Yamaguchi et al.
Langmuir : the ACS journal of surfaces and colloids, 27(20), 12521-12532 (2011-09-09)
Micropatterning techniques have become increasingly important in cellular biology. Cell patterning is achieved by various methods. Photolithography is one of the most popular methods, and several light sources (e.g., excimer lasers and mercury lamps) are used for that purpose. Vacuum
Allyson E Sgro et al.
Journal of neuroscience methods, 198(2), 230-235 (2011-04-26)
Generating microislands of culture substrate on coverslips by spray application of poly-d lysine is a commonly used method for culturing isolated neurons that form self (autaptic) synapses. This preparation has multiple advantages for studying synaptic transmission in isolation; however, generating
Kyungtae Kang et al.
Biomaterials, 32(27), 6374-6380 (2011-06-10)
Chemical surface modification of neuron-surface interfaces is essential for the development of biologically active and functional neural interfaces. Different types of surface modification schemes are required to derivatize either electrode or insulator surfaces, which limits the surface chemistry based neural
Yong Hee Kim et al.
Journal of neuroscience methods, 202(1), 38-44 (2011-09-13)
We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite
Roberto de la Rica et al.
Analytical chemistry, 81(24), 10167-10171 (2009-11-17)
While cancer is still an implacable disease, many cancers can be cured if they are diagnosed in an early stage. Recently, it was reported that the transformation from normal cells to cancer cells can change their mechanoelastic properties to become
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