无菌性
non-sterile
质量水平
形式
buffered aqueous solution
浓度
2 mg/mL
技术
capture ELISA: 5 μg/mL using Platelet extract (Use Sigma Monoclonal Anti-Phosphotyrosine Antibody (Prod. No. P3300))
western blot: 5 μg/mL using Platelet extract (Use Sigma Monoclonal Anti-Phosphotyrosine P-3300)
运输
dry ice
储存温度
−20°C
一般描述
The activity of proteins depends on phosphorylation of specific amino acid residues. In applications involving identification of activated cellular proteins, it is important to confirm specificity of antibodies used against a particular phosphorylated amino acid. For such applications, inhibitors of phosphorylated antibodies on specific amino acid residues are suitable. Phosphotyrosine antibody inhibitor may be used for the specific inhibition of the reactivity of anti-phosphotyrosine antibodies but not that of anti-phosphothreonine- or anti-phosphoserine-specific antibodies.
特异性
Phosphotyrosine antibody inhibitor that specifically blocks the reactivity of anti-phosphotyrosine antibody. It does not block the activity of anti-phosphothreonine or anti-phosphoserine-specific antibodies.
应用
Phosphotyrosine-BSA may be used for specific inhibition of phosphotyrosine in platelet extracts, in immunoblotting and ELISA applications, at a working concentration of 5 μg/mL.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
分析说明
O-Phospho-L-tyrosine conjugated to bovine serum albumin
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Birte Schulenberg et al.
Electrophoresis, 25(15), 2526-2532 (2004-08-10)
Protein phosphorylation plays a vital role in the regulation of most aspects of cellular activity, being key to propagating messages within signal transduction pathways and to modulating protein function. Pro-Q Diamond phosphoprotein gel stain is suitable for the fluorescence detection
Motoyuki Ogawa et al.
Biochemical and biophysical research communications, 304(4), 676-683 (2003-05-03)
Paxillin has been recognized as a focal adhesion adapter protein that participates in the integrin-mediated signaling. An earlier study [Ogawa et al. Biochim. Biophys. Acta 1519 (2001) 235] found that frog paxillin was expressed in the kidney epithelial cell line
Jacqueline Fischer-Lougheed et al.
Immunology, 123(3), 390-397 (2007-10-06)
Synthetic anti-idiotypic antibodies represent a potentially valuable tool for the isolation and characterization of B cells that produce xenoantibodies. An anti-idiotypic antibody that binds to a subset of B cells producing antibodies encoded by the variable-region heavy chain 3 (V(H)3)
Zhihong Zeng et al.
The Journal of biological chemistry, 286(1), 403-409 (2010-10-30)
Topoisomerase II (Top2) activity involves an intermediate in which the topoisomerase is covalently bound to a DNA double-strand break via a 5'-phosphotyrosyl bond. Although these intermediates are normally transient, they can be stabilized by antitumor agents that act as Top2
Matthew G Vander Heiden et al.
Science (New York, N.Y.), 329(5998), 1492-1499 (2010-09-18)
Proliferating cells, including cancer cells, require altered metabolism to efficiently incorporate nutrients such as glucose into biomass. The M2 isoform of pyruvate kinase (PKM2) promotes the metabolism of glucose by aerobic glycolysis and contributes to anabolic metabolism. Paradoxically, decreased pyruvate
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