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Merck
CN
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P3288

Sigma-Aldrich

磷酸盐缓冲液，25 °C，pH 7.2

for hematology and histology staining techniques

别名:

磷酸缓冲液

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About This Item

UNSPSC代码:
12352107
NACRES:
NA.47

一般描述

磷酸盐缓冲液是手术灌洗液的最常用组分，用于保存活组织。它也用于免疫化学染色流程。磷酸盐缓冲液是光学和电子显微镜的重要固定剂。其缓冲能力对pH敏感，最好在生理pH（7.2-7.4）下使用。

应用

适合用作各种Romanowsky型染色流程的缓冲液，包括Wright Stain、Wright Giemsa、Giemsa、May Grunwald、Jenner和Leishman。在25℃下pH 7.2的磷酸盐缓冲液已被用于收集猪卵巢的卵母细胞。

重悬

每瓶可制备3.8 L缓冲液，8.3 mM。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How to solubilize Product P3288, Phosphate Buffer pH 7.2?

    The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.

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