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表单
lyophilized powder
比活
~0.2 units/mg protein
分子量
~60 kDa
组成
Protein, ~80%
UniProt登记号
储存温度
−20°C
基因信息
cow ... PDE1A(281969)
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应用
For assay of 3′,5′-cyclic nucleotides.
生化/生理作用
水解环单磷酸核苷酸（如cAMP 和 cGMP）的 3′,5′-磷酸二酯，生成相应的 5′-单磷酸核苷酸。
质量
Contains inorganic pyrophosphatase and 5′-ATPase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
单位定义
One unit will hydrolyze 1.0μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.
外形
Lyophilized preparation containing near saturation levels of both protein activator and Ca++, and buffer salts as imidazole and magnesium sulfate
制备说明
Prepared as described by Butcher, R.W. and Sutherland, E.W., J. Biol. Chem., 237, 1244 (1962).
重悬
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. Both the activated and activator deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.
分析说明
除非特别说明，否则均采用 Warburg-Christian 法测定蛋白质。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
The cyclic AMP pathway.
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PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3'5' adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but
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The Journal of clinical endocrinology and metabolism, 97(12), 4355-4357 (2012-12-12)
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