一般描述
GenElute 细菌基因组DNA试剂盒可以简单、方便地从革兰氏阴性菌中分离高纯度基因组DNA。对于大多数革兰氏阳性菌，本试剂盒必须结合选购的溶菌酶 (L4919)来有效裂解较厚的肽聚糖细胞壁。为方便您制备溶菌酶储液，我们提供了一种稀释的革兰氏阳性溶解溶液。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
该试剂盒将硅胶膜系统的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。
应用
纯化所得的细菌基因组DNA可用于以下游应用：
- 限制性内切酶酶切
- PCR
- Southern印迹
- 克隆
特点和优势
- 起始材料：不超过1.5 mL培养物
- 预期得率：至多20 μg
- 洗脱体积：400 μl
- 所需时间：70 - 120分钟
- A260/A280比率：1.6 - 1.9
- 无需苯酚、氯仿或乙醇沉淀步骤
原理
细菌首先在含有离液盐的溶液之中裂解，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
预期的基因组DNA得率取决于细菌培养物的细胞密度、细菌种属以及所用的菌株。通过GenElute试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
其他说明
更多信息，请见www.sigma-aldrich.com/genomicdna。
法律信息
GenElute is a trademark of Sigma-Aldrich Co. LLC
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警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Central nervous system, Respiratory system
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实验方案
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。
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