生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
NB2, monoclonal
分子量
antigen 500 kDa
包含
15 mM sodium azide
种属反应性
vertebrates, chicken
技术
indirect immunofluorescence: 1:400 using frozen sections of animal skeletal muscle
western blot: suitable
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
chicken ... NEB(101748402)
一般描述
Nebulin is a 700kDa protein which is expressed in skeletal muscles. It possesses 35 amino acid repeats and a C terminal serine-rich and SH3 domains.
Nebulin is also localized to cardiac muscle. Monoclonal Anti-Nebulin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
特异性
Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
免疫原
chicken breast nebulin.
应用
Monoclonal Anti-Nebulin antibody produced in mouse has been used in:
- immunohistochemistry
- fluorescent immunostaining
- western blotting
生化/生理作用
Nebulin associates with the Z-line and thin actin filament in a sarcomere. It may function as a regulator of sarcomere thin filament length. The protein also acts as a structural link between sarcomeres. Nebulin interacts with the capping protein CapZ and has a role in controlling the localization of actin filaments.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Identification of chicken nebulin isoforms of the 31-residue motifs and non-muscle nebulin.
Joo YM
Biochemical and Biophysical Research Communications (2004)
Clinical phenotype, muscle MRI and muscle pathology of LGMD1F
Peterle E, et al.
Journal of Neurology, 260(8), 2033-2041 (2013)
J Q Zhang et al.
Biophysical journal, 74(1), 349-359 (1998-02-04)
cDNA clones encoding mouse skeletal muscle nebulin were expressed in Escherichia coli as thioredoxin fusion proteins and purified in the presence of 6 M urea. These fragments, called 7a and 8c, contain 28 and 19 of the weakly repeating approximately
Biallelic mutations in MYPN, encoding myopalladin, are associated with childhood-onset, slowly progressive nemaline myopathy
Miyatake S, et al.
American Journal of Human Genetics, 100(1), 169-178 (2017)
Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
Pappas CT
Molecular Biology of the Cell (2008)
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