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安全信息

N7271

Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

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别名:
受体破坏酶, 唾液酸酶, 神经氨酸苷酶
CAS号:
9001-67-6
EC 号:
3.2.1.18 (BRENDA, IUBMB)
MDL编号:
MFCD01092203
UNSPSC代码:
12352204
NACRES:
NA.32

生物来源

Streptococcus pneumoniae

质量水平

100

形式

buffered aqueous solution

浓度

≥5 units/mL

异质活性

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

运输

wet ice

储存温度

2-8°C

基因信息

Streptococcus pneumoniae R6 ... nanB(933504)

相关类别

生化/生理作用

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

包装

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

单位定义

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

外形

Solution in 50 mM sodium phosphate, pH 7.5

制备说明

Expressed in glycosidase-free hosts.

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

  6. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

  7. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

  8. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Nature, 508(7494), 61-65 (2014-02-28)
Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures
Audu J Natala et al.
Journal of medical entomology, 50(1), 85-93 (2013-02-23)
Amblyomma variegatum F. are obligate hematophagous ectoparasites of livestock that serve as the vectors of Ehrlichia ruminantium (formerly known as Cowdria ruminantium), the causative agent of heartwater disease. In the light of the fact that they are blood-feeding, their salivary
Johan Nordholm et al.
The Journal of biological chemistry, 288(15), 10652-10660 (2013-03-01)
Interactions that facilitate transmembrane domain (TMD) dimerization have been identified mainly using synthetic TMDs. Here, we investigated how inherent properties within natural TMDs modulate their interaction strength by exploiting the sequence variation in the nine neuraminidase subtypes (N1-N9) and the
Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Chinh Tran-To Su et al.
BMC bioinformatics, 14 Suppl 16, S7-S7 (2014-02-26)
Since late March 2013, there has been another global health concern with a sudden wave of flu infections by a novel strain of avian influenza A (H7N9) virus in China. To-date, there have been more than 100 infections with 23
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