生物来源
Staphylococcus aureus
质量水平
形式
lyophilized powder
比活
100-300 units/mg protein
组成
Protein, ≥40% E1%/280 (Balance primarily sodium citrate)
技术
DNA purification: suitable
适用性
suitable for molecular biology
应用
diagnostic assay manufacturing
储存温度
−20°C
基因信息
Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)
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相关类别
应用
来自 金黄色葡萄球菌 的核酸酶已被用于评估动物中发现的凝固酶和耐热菌株的研究中。 它也被用于研究 金黄色葡萄球菌 中编码两个热稳定核酸酶基因的表达特征。
生化/生理作用
可水解DNA的5′-磷酸二酯键。
相较于G或C侧，微球菌核酸酶可在A或T核苷酸的5′ 侧好的对DNA进行切割。
单位定义
在pH 8.8，37℃条件下，一个单元可每分钟从天然DNA产生1.0μmole的酸可溶性多核苷酸，对于混合核苷酸来说基于EM/260 = 10,000 。
其他说明
注意：1μmole单位 = 约85 A260 单位，其中A260单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA260为1.0。光路= 1cm。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Yu Hu et al.
Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)
Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases
C Dingwall et al.
Nucleic acids research, 9(12), 2659-2673 (1981-06-25)
The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'
Gang Wei et al.
Methods in enzymology, 513, 297-313 (2012-08-30)
Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features.
Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals
DEVRIESE, L. and P. OEDING
Journal of Applied Microbiology, 39, 197-207 (1975)
Julien Roche et al.
Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)
The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)
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