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N3755

微球菌核酸酶来源于金黄色葡萄球菌

Name: 微球菌核酸酶 来源于<I>金黄色葡萄球菌</I>
Brand: SIGMA

100-300 units/mg protein

别名:

微球菌核酸酶, MNase, 微球菌核酸内切酶

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About This Item

CAS号:

9013-53-0

EC 号:

3.1.31.1 (BRENDA, IUBMB)

EC 号:

232-748-0

MDL编号:

MFCD00131731

UNSPSC代码:

12352204

NACRES:

NA.54

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1 of 4

此商品	28891U	29479U	29484U
28880U SLB^®-IL59 毛细管气相色谱柱	28891U SLB^®-IL59 毛细管气相色谱柱	29479U SLB^®-IL82 毛细管气相色谱柱	29484U SLB^®-IL61 Capillary GC Column
matrix active group Non-bonded; 1,12-Di(tripropylphosphonium)dodecane bis(trifluoromethanesulfonyl)imide phase	matrix active group Non-bonded; 1,12-Di(tripropylphosphonium)dodecane bis(trifluoromethanesulfonyl)imide phase	matrix active group Non-bonded; 1,12-Di(2,3-dimethylimidazolium)dodecane bis(trifluoromethanesulfonyl)imide phase	matrix active group Non-bonded; 1,12-Di(tripropylphosphonium)dodecane bis(trifluoromethanesulfonyl)imide trifluoromethanesulfonate phase
parameter ≤25-300 °C temperature (isothermal or programmed)	parameter ≤25-300 °C temperature (isothermal or programmed)	parameter 50-270 °C temperature (isothermal or programmed)	parameter 40-290 °C temperature (isothermal or programmed)
application(s) agriculture chemicals and industrial polymers clinical environmental food and beverages forensics and toxicology petroleum	application(s) agriculture chemicals and industrial polymers clinical environmental food and beverages forensics and toxicology petroleum	application(s) environmental	application(s) -
technique(s) gas chromatography (GC): suitable (fast GC)	technique(s) gas chromatography (GC): suitable	technique(s) gas chromatography (GC): suitable	technique(s) gas chromatography (GC): suitable (fast GC)
material fused silica	material fused silica	material fused silica	material fused silica

应用

来自 金黄色葡萄球菌 的核酸酶已被用于评估动物中发现的凝固酶和耐热菌株的研究中。 [1] 它也被用于研究 金黄色葡萄球菌 中编码两个热稳定核酸酶基因的表达特征。 [2]

生化/生理作用

可水解DNA的5′-磷酸二酯键。

相较于G或C侧，微球菌核酸酶可在A或T核苷酸的5′ 侧好的对DNA进行切割。 [3]

单位定义

在pH 8.8，37℃条件下，一个单元可每分钟从天然DNA产生1.0μmole的酸可溶性多核苷酸，对于混合核苷酸来说基于EM/260 = 10,000 。

其他说明

注意：1μmole单位 = 约85 A₂₆₀ 单位，其中A₂₆₀单位相当于在pH 8.8，37℃条件下，在3.55 mL反应体积中，30分钟内ΔA₂₆₀为1.0。光路= 1cm。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

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Coagulase and Heat-resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

DEVRIESE, L. and P. OEDING

Journal of Applied Microbiology, 39, 197-207 (1975)

High sequence specificity of micrococcal nuclease.

C Dingwall et al.

Nucleic acids research, 9(12), 2659-2673 (1981-06-25)

The substrate specificity of micrococcal nuclease (EC 3.1.4.7.) has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5'

Comparative expression analysis of two thermostable nuclease genes in Staphylococcus aureus.

Yu Hu et al.

Foodborne pathogens and disease, 9(3), 265-271 (2012-03-03)

Thermonuclease is known as a specific virulence factor in Staphylococcus aureus. It is widely used as a genetic marker for detection of S. aureus in various types of food. Previous studies have revealed the existence of two functional thermostable nucleases

Genome-wide mapping of nucleosome occupancy, histone modifications, and gene expression using next-generation sequencing technology.

Gang Wei et al.

Methods in enzymology, 513, 297-313 (2012-08-30)

Gene transcription can be regulated through alteration of chromatin structure, such as changes in nucleosome positioning and histone-modification patterns. Recent development of techniques based on the next-generation sequencing technology has allowed high-resolution analysis of genome-wide distribution of these chromatin features.

Effect of internal cavities on folding rates and routes revealed by real-time pressure-jump NMR spectroscopy.

Julien Roche et al.

Journal of the American Chemical Society, 135(39), 14610-14618 (2013-08-31)

The time required to fold proteins usually increases significantly under conditions of high pressure. Taking advantage of this general property of proteins, we combined P-jump experiments with NMR spectroscopy to examine in detail the folding reaction of staphylococcal nuclease (SNase)

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微球菌核酸酶来源于金黄色葡萄球菌