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等级
for molecular biology
质量水平
无菌性
non-sterile
形式
powder
组成
MgSO4, 1 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
pH值（酸碱度）
6.3-7.2(1.6% solution)
应用
food and beverages
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
应用
Suitable for non-selective cultivation of lambda bacteriophage.
特点和优势
NZ Amine powder provides:
- Standard formulation
- Convenient package sizes
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
制备说明
1. Suspend 15.94 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
重悬
Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
法律信息
N-Z-Amine is a registered trademark of Kerry Group
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Difco Manual, 356-356 (1998)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, A-A (1989)
F R Blattner et al.
Science (New York, N.Y.), 196(4286), 161-169 (1977-04-08)
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector
实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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