产品名称
NZ Amine® Broth, Lambda bacteriophage growth medium
grade
Molecular Biology
sterility
non-sterile
form
powder
composition
MgSO4, 1 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
pH
6.3-7.2(1.6% solution)
application(s)
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Quality Level
Application
Suitable for non-selective cultivation of lambda bacteriophage.
Features and Benefits
NZ Amine powder provides:
- Standard formulation
- Convenient package sizes
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
General description
NZ Amine is a growth medium used for the cultivation of lambda bacteriophage. This nutrient-rich broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Preparation Note
1. Suspend 15.94 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
Stir to suspend 16 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Legal Information
N-Z-Amine is a registered trademark of Kerry Group
存储类别
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
Difco Manual, 356-356 (1998)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, A-A (1989)
F R Blattner et al.
Science (New York, N.Y.), 196(4286), 161-169 (1977-04-08)
The Charon lambda bacteriophages have been developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector
实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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