推荐产品
产品名称
4-硝基苯磷酸盐 二(三羟甲基氨基甲烷) 盐, phosphatase substrate
质量水平
方案
≥97% (enzymatic)
表单
powder
溶解性
water: 50 mg/mL, clear, colorless to light yellow
储存温度
−20°C
SMILES字符串
NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
InChI
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
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应用
4-硝基苯磷酸盐双(三羟甲基氨基甲烷)可用于：
- 作为底物定量分析细胞裂解液中的抗酒石酸酸性磷酸酶(TRAP)活性
- 天然聚丙烯酰胺凝胶电泳(PAGE)测定磷酸酶活性
- 在酶联免疫吸附测定(ELISA)中用于碱性磷酸酶
生化/生理作用
对硝基苯磷酸(pNPP)具有高灵敏性，在酶联免疫吸附测定(ELISA)中特别适合作为碱性磷酸酶作用底物。该底物反应生成可溶性黄色终产物，可在405nm处通过分光光度法读取。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
B Le Gars Santoni et al.
Bioactive materials, 10, 222-235 (2021-12-14)
β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on
Julieta B Carrillo et al.
PloS one, 13(1), e0191621-e0191621 (2018-01-24)
Ostreococcus tauri, the smallest free-living (non-symbiotic) eukaryote yet described, is a unicellular green alga of the Prasinophyceae family. It has a very simple cellular organization and presents a unique starch granule and chloroplast. However, its starch metabolism exhibits a complexity
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
B Le Gars Santoni et al.
Bioactive materials, 10, 222-235 (2021-12-14)
β-Tricalcium Phosphate (β-TCP), one of the most used bone graft substitutes, may contain up to 5 wt% foreign phase according to standards. Typical foreign phases include β-calcium pyrophosphate (β-CPP) and hydroxyapatite (HA). Currently, the effect of small amounts of impurities on
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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