N2765
4-硝基苯基磷酸盐 二钠盐 六水合物
chromogenic, tablet
别名:
4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物
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关于此项目
线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
化学文摘社编号:
分子量:
371.14
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
224-246-5
MDL number:
Beilstein/REAXYS Number:
5324661
产品名称
4-硝基苯基磷酸盐 二钠盐 六水合物, tablet
form
tablet
Quality Level
solubility
water, high purity: 0.25 tablet/mL, clear, colorless
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
General description
用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应，并在 405nm 下读数。
Application
4-硝基苯磷酸二钠盐六水合物已用于：
- 在酶联免疫吸附测定(ELISA)中作为碱性磷酸酶偶联抗兔抗体的底物
- 在酶标免疫实验中作为碱性磷酸酶标记小鼠二抗的底物
- ELISA法分析动物血清样本中铜绿假单胞菌抗体的存在
Preparation Note
每片含有20mg底物
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| N2765-50TAB | 04061834110477 |
| N2765-100TAB | 04061835546237 |
| N2765-5X100TAB | 04061834110484 |