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Merck
CN

N2640

Sigma-Aldrich

4-硝基苯基磷酸盐 二钠盐 六水合物

chromogenic, tablet

别名:

4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物

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About This Item

线性分子式:
O2NC6H4OP(O)(ONa)2 · 6H2O
分子量:
371.14
Beilstein:
5324661
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

产品名称

4-硝基苯基磷酸盐 二钠盐 六水合物, tablet

表单

tablet

质量水平

溶解性

water: 1 tablet/3 mL, clear, colorless

储存温度

−20°C

SMILES字符串

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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相关类别

一般描述

用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。

应用

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.

生化/生理作用

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.

数量

Contains 15 mg substrate per tablet

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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访问文档库

Christopher J Sample et al.
Peptides, 48, 96-105 (2013-07-31)
Broad-spectrum antiviral drugs are urgently needed to treat individuals infected with new and re-emerging viruses, or with viruses that have developed resistance to antiviral therapies. Mammalian natural host defense peptides (mNHP) are short, usually cationic, peptides that have direct antimicrobial
Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines
Penkert RR, et al.
Cytokine, 91, 1-5 (2017)
Alkaline Phosphatase Assay Based on the Chromogenic Interaction of Diethanolamine with 4-Aminophenol
Sun J, et al.
Analytical Chemistry, 90(10), 6339-6345 (2018)
Protein tyrosine phosphatase assays
Lorenz U
Current Protocols in Immunology, 93(1), 11-17 (2011)
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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