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Merck
CN
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文件

N1630

Sigma-Aldrich

β-烟酰胺腺嘌呤二核苷酸2′-磷酸，还原型 四钠盐 水合物

≥93% (HPLC)

别名:

β-NADPH, 2′-NADPH 水合物, NADPH Na 4, TPNH2 Na4, 磷酸二氢烟酰胺腺嘌呤二核苷酸 四钠盐, 还原型三磷酸吡啶核苷酸 四钠盐, 还原型辅酶 II 四钠盐

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About This Item

经验公式（希尔记法）:
C21H26N7Na4O17P3 · xH2O
CAS号:
2646-71-1
分子量:
833.35 (anhydrous basis)
EC 号:
220-163-3
MDL编号:
MFCD00036263
UNSPSC代码:
41106305
PubChem化学物质编号:
24897509
NACRES:
NA.51

质量水平

300

检测方案

≥93% (HPLC)
≥93% (spectrophotometric assay)

形式

powder

溶解性

10 mM NaOH: soluble 50 mg/mL, clear

储存温度

−20°C

SMILES字符串

[Na+].[Na+].[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@H]2O[C@@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](OP([O-])([O-])=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@H](O)[C@@H]2O

InChI

1S/C21H30N7O17P3.4Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;;;/h1,3-4,7-8,10-11,13-16,20-21,29-31H,2,5-6H2,(H2,23,32)(H,36,37)(H,38,39)(H2,22,24,25)(H2,33,34,35);;;;/q;4*+1/p-4/t10-,11+,13-,14+,15-,16+,20-,21+;;;;/m0..../s1

InChI key

WYWWVJHQDVCHKF-MPUNMZHWSA-J

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相关类别

应用

β-NADPH适用于：
  • 脂肪酸去饱和
  • 去饱和酶活性检测
  • 大鼠、兔和雉鸡胸脊髓中NADPH黄递酶活性的组化检测
  • 少突胶质细胞培养物中的谷胱甘肽检测

生化/生理作用

ββ--NADPH是由磷酸戊糖途径产生的还原形式的NADP+。作为还原力来源，在生物合成的还原反应中将H+和2 e– 转移至氧化前体中。它是谷胱甘肽还原酶、二乙酰还原酶、二氢叶酸还原酶、谷氨酸脱氢酶、对羟基苯甲酸羟化酶、NADPH-FMN氧化还原酶、硝酸还原酶和硫氧还蛋白还原酶等酶发挥催化活性的必需辅酶。它也是细胞色素P450 电子传递系统的一部分。
NADPH是许多氧化还原酶(包括一氧化氮合成酶)的电子供体和辅助因子。

制备说明

化学还原。

其他说明

根据固体重量包装。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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D Kluchová et al.
European journal of histochemistry : EJH, 45(3), 239-248 (2002-01-05)
The distribution of NADPH-diaphorase (NADPH-d) activity was investigated and compared in the rat, rabbit and pheasant thoracic spinal cord. The investigation of all spinal cord regions (laminae) in three experimental species revealed marked differences in the distribution of NADPH-d activity.
H Wada et al.
Journal of bacteriology, 175(18), 6056-6058 (1993-09-01)
The desA gene of the cyanobacterium Synechocystis sp. strain PCC6803 was expressed in Escherichia coli, which does not contain any fatty acid desaturase. The product of the desA gene catalyzed the desaturation of fatty acids at the delta 12 position.
H Wada et al.
Journal of bacteriology, 175(2), 544-547 (1993-01-01)
Thylakoid membranes isolated from the cyanobacterium Synechocystis sp. strain PCC6803 were capable of desaturating the acyl groups in monogalactosyl diacylglycerol. This desaturation reaction required the reduced form of ferredoxin.
William B. Wood
Biochemistry: A Problems Approach, 195-195 null
S A Back et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 18(16), 6241-6253 (1998-08-11)
Death of oligodendrocyte (OL) precursors can be triggered in vitro by cystine deprivation, a form of oxidative stress that involves depletion of intracellular glutathione. We report here that OLs demonstrate maturation-dependent differences in survival when subjected to free radical-mediated injury
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实验方案

Enzymatic Assay of Ferredoxin NADP Reductase (EC 1.18.12)

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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